Biotek Synergy HTX, Operator'S Manual, Page: 117 / 184

System Test |
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System Test
Each time the Synergy HTX is turned on, it automatically performs a series of tests on the
reader’s motors, lamp, the PMT, and various subsystems. The duration of this system test
depends on the reader model, and can take a few minutes to complete. If all tests pass, the
microplate carrier is ejected and the LED on the power switch remains on.
If any test results do not meet the internally coded Failure Mode Effects Analysis (FMEA)
criteria established by BioTek, the reader beeps repeatedly. If this occurs, press the carrier
eject button to stop the beeping. If necessary, initiate another system test using Gen5 to try
to retrieve an error code from the reader.
Refer to
Appendix B, Error Codes
for information on error codes and for
troubleshooting tips.

The system test runs automatically when the instrument is turned on.
If this “power-up” system test fails, the instrument will beep
repeatedly. If this happens, press the carrier eject button to stop the
beeping and then initiate a system test through Gen5 to retrieve the
error code.
1.
If applicable, adjust Gen5’s Absorbance Wavelength table to values that will
confirm operation of the reader at its limits. We recommend 200 and 999 nm
(the lower and upper limits of the monochromator) and any four
wavelengths in between that best represent your assays and/or the lowest
and highest wavelength values used in your lab.
• From Gen5’s main screen, select
System > Instrument
Configuration
. Highlight
Synergy HTX
and click
View/Modify
.
•
Click
Setup
and then click the
Absorbance
tab. The six wavelength
values currently in use are displayed.
•
Enter the six wavelength values you wish to test and then click
Send
Wavelengths
to download them to the Synergy HTX.
2.
If the reader is equipped with an incubator, select
System > Instrument
Control > Synergy HTX
and click the
Pre-Heating
tab. Enter a
Requested
temperature of at least 37°C and then click
On
. Wait until the
temperature reaches the set point before continuing.
3.
Return to Gen5’s main screen. Select
System > Diagnostics > Run
System Test
.

If the test fails during execution, a message box will appear in the
software. Close the box; the test report will contain the error code
that was generated by the failure.
4.
When the test is complete, a dialog will appear, requesting additional
information. Enter the information (if required) and then click
OK
.
Synergy HTX Operator's Manual