Biotek Synergy HTX, Operator'S Manual, Page: 71 / 184

Recommendations for Optimum Performance |
49
• To keep the dispense system in top condition, flush and purge the fluid lines with
deionized (DI) water every day or upon completion of an assay run, whichever is
more frequent. Some reagents may crystallize or harden after use, clogging the
fluid passageways. Flushing the tubing at the end of each day, letting the DI water
soak them overnight, and then purging the lines at the beginning of each day
ensures optimal performance of the dispense system. See
Chapter 4, Preventive
Maintenance
for more information.
•
For models with injectors:
When dispensing volumes less than or equal to 20
µL/well, we recommend specifying a tip prime volume that is equal to the
dispense volume. For dispense volumes greater than 20 µL/well, we recommend a
tip prime volume of 20 µL.
Incubation and Partial Plates
When performing a partial plate read that includes an incubation step, the following
recommendations can reduce the effects of evaporation of your samples:
•
Use microplate lids.
• Fill unused wells with fluid.
• Cluster your sample wells rather than spacing them throughout the plate.
•
Place your sample wells in the center of the plate. This placement may lead to less
evaporation than if you place the samples in wells on the edge of the plate.
Synergy HTX Operator's Manual