Biotek Synergy HTX, Operator'S Manual, Page: 152 / 184

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Chapter 6: Instrument Qualification
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A
B
C 100 nM 50 nM 25 nM 12.5 nM 6.25 nM BUF
D 100 nM 50 nM 25 nM 12.5 nM 6.25 nM
MUB
100 nM
BUF
E 100 nM 50 nM 25 nM 12.5 nM 6.25 nM BUF
F 100 nM 50 nM 25 nM 12.5 nM 6.25 nM
G
H
Protocol Reading Parameters

Your tests may require modifications to some of the parameters below,
such as Plate Type or Gain (see “Troubleshooting” on page 120). The
Plate Type setting should match the plate you are actually using.
The following table contains the recommended settings for the
Synergy HTX
FI_MUB.prt
protocol.
Protocol Name: Synergy HTX FI_ MUB.prt
Parameter Default Setting
Plate Type: Costar 96 black opaque (Mfr. #3915)
Read Step 1
Kinetic Loop:
Run time :45 seconds
Interval: 3 seconds
Reads: 16
Read Step Label: “Sensitivity Read”
Read Well: D7
Filter Set: EX 360/40 nm
EM 460/40 nm
BioTek Instruments, Inc.