Biotek Synergy HTX, Operator'S Manual, Page: 162 / 184

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Chapter 6: Instrument Qualification
dispenser. You will create two Gen5 protocols to perform the dispense steps. If you will
use a BioTek absorbance reader that is supposed by Gen5, you will create one additional
protocol to perform the Read step.
Create the Dispense Protocols

Perform these steps to create a protocol to test Dispenser 1. Then,
open a copy of the protocol and change the relevant Procedure
parameters to Dispenser 2.
1.
In Gen5, create a new protocol.
2.
Define the procedure with the steps and settings as described in this table:
# Step Type Details
1 Dispense
Dispenser <select 1 or 2, depending on the
protocol>
Dispense to wells A1..H4
Tip prime before this dispense step, 20 µL
Dispense 80 µL at rate 275 µL/sec
2 Plate Out,In Suggested comment: Weigh the plate (80 uL test).
RECORD the weight, TARE the balance. Place the
plate back on the carrier. Click OK to continue.
3 Dispense
Dispenser <select 1 or 2, depending on the
protocol>
Dispense to wells A5..H8
Tip prime before this dispense step, 20 µL
Dispense 20 µL at rate 250 µL/sec
4 Plate Out,In Suggested comment: Weigh the plate (20 uL test).
RECORD the weight, TARE the balance. Place the
plate back on the carrier. Click OK to continue.
5 Dispense
Dispenser <select 1 or 2, depending on the
protocol>
Dispense to wells A9..H12
Tip prime before this dispense step, 5 µL
Dispense 5 µL at rate 225 µL/sec
6 Plate Out,In Suggested comment: Weigh the plate (5 uL test).
RECORD the weight. Set the plate aside and click
OK.
7 Read
Wells: A1
Detection Method: <select any valid method>
Read Type:
Endpoint
Read Speed: Normal
Wavelength: <select any valid wavelength(s)>
The Read step is necessary because Gen5 requires a Read step within any
Dispense procedure. When the test is run, the measurement value is not
used.
BioTek Instruments, Inc.