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Chapter 6: Instrument Qualification
Protocol Name: Synergy HTX FI_T.prt
Parameter
Default Setting
Optics Position:
Top
Gain:
Auto
Scale to High Wells
Scale Well: A3
Scale Value: 30000
Delay after Plate Movement:
100 msec
Measurements per Data Point:
50
Dynamic Range:
Standard
Light Source:
Tungsten
Read Height:
1.00 mm
Read Step 4
Read Step Label:
“Linearity Read”
Read Wells:
C1–F5
Filter Set:
EX 485/20 nm
EM 528/20 nm
Optics Position:
Top
Gain:
Auto
Scale to High Wells
Scale Well: C1
Scale Value: 30000
Delay after Plate Movement:
100 msec
Measurements per Data Point:
50
Dynamic Range:
Standard
Light Source:
Tungsten
Fluorescence Tests Using Methylumbelliferone
As an alternative to using Sodium Fluorescein, Methylumbelliferone (“MUB”) can be
used to test the
top optics
for the fluorescence system.
Required Materials
BioTek offers a liquid test kit (PN 7160012) containing the microplate
and solutions used in this procedure.
Microplates should be perfectly clean and free of dust or bottom
scratches. Use new microplates from sealed packages.
Manufacturer part numbers are subject to change.
BioTek Instruments, Inc.
Summary of Contents for Synergy HTX
Page 1: ...Operator s Manual Multi Mode Microplate Reader Synergy HTX ...
Page 2: ......
Page 22: ...xviii Preface BioTek Instruments Inc ...
Page 28: ...6 Chapter 1 Introduction BioTek Instruments Inc ...
Page 52: ...30 Chapter 2 Installation BioTek Instruments Inc ...
Page 72: ...50 Chapter 3 Getting Started BioTek Instruments Inc ...
Page 102: ...80 Chapter 4 Preventive Maintenance BioTek Instruments Inc ...
Page 166: ......
Page 174: ...150 Appendix A Specifications BioTek Instruments Inc ...