Biotek Synergy HTX, Operator'S Manual

Page: 64 / 184

42
|
Chapter 3: Getting Started
values, print /export results, and more. This section provides brief instructions for
creating experiments and reading plates. It also explains how to use Gen5 to perform some
functions that are specific to the dispense module.
Viewing/Updating the Filter and Wavelengths Tables
If configured with fluorescence or luminescence capability, the Synergy HTX ships
with a set of excitation and emission filters installed, and the reader’s onboard software
is preconfigured with the filter values and their locations. When Gen5 establishes
communication with the reader, it “asks” for this information and then stores it in a
filter table on the computer.
To view this table in Gen5, select
System > Instrument Configuration
, highlight
the Synergy HTX reader, and click
View/Modify.
Click
Setup
and then click the
Fluorescence/Luminescence
tab.
Regarding the
Absorbance
Wavelengths table:
The Synergy HTX
performs absorbance
reads in the range of
200 to 999 nm.
Click the Absorbance
tab to specify and
calibrate 6 wavelengths
to be made available as
default selections within
a protocol’s Reading
Parameters dialog.
To change the settings and download them to the instrument:
1.
Select
Band Pass
,
Plug
, or
Hole
for the excitation and emission filter
wheels.
2.
For each filter type, enter the wavelength value and its accompanying
bandwidth. (The bandwidth is printed on the side of each filter.)
3.
When finished, click
Send Values
to download the information to the
reader. (Clicking
Get Values
uploads information from the reader.)
4.
Click
OK
to save the settings and close this dialog. The settings become
available for selection in the Read step dialog in a Procedure.
BioTek Instruments, Inc.