Biotek Synergy HTX, Operator'S Manual, Page: 161 / 184

Dispense Module Tests |
139
3.
Create
Data Reduction
steps to calculate Delta OD values:
• Select
Protocol > Data Reduction
and select
Custom
.
•
Within this dialog, click the
Select Multiple Data Sets
button and then
click the
DS2
radio button.

Set the
Data In
for
DS1
to the
80
µL Read step at
405
nm.

Set the
Data In
for
DS2
to the
80
µL Read step at
750
nm.
• Click
OK
to return to the dialog.
•
In the
New Data Set Name
field, type an identifying name such as ‘Delta
OD 80 uL_Disp 1’.
• Clear
Use single formula for all wells.
•
In the
Current Formula
field, type
DS1-DS2
and then assign the formula
to wells
A1
to
H4
.
• Click
OK
to add the transformation to the Data Reduction list.
•
Create another Transformation similar to the above, with these
characteristics:
 DS1
set to the
20 and 5
µL Read step at
630
nm

DS2
set to the
20 and 5
µL Read step at
750
nm
 New Data Set Name
resembling ‘Delta OD 20 and 5 uL_Disp <#>’

Formula
DS1-DS2
applied to wells
A5
to
H12
4.
(This step is optional.) The results analysis worksheet at the end of this chapter
requires the calculation of the Standard Deviation, Mean, and % CV of the ODs
read for each dispense volume in each plate (six sets of calculations). By
identifying the wells by their dispense volumes in the Plate Layout, Gen5 will
calculate these values for you.
•
Open the Plate Layout dialog.
• Define three Assay Control IDs and assign them to the following wells:
 Disp_80
A1 to H4

Disp_20
A5 to H8

Disp_5
A9 to H12

After running the experiment, view the Statistics for each Delta OD
Data Set to view the calculations.
5.
Save the protocols as
Synergy HTX Disp 1 Test.prt
and
Synergy HTX Disp
2 Test.prt
.
Gen5 Test Protocols for Models without Absorbance Capabilities
The test procedure on page 135 dispenses three volumes of fluid to a microplate and then
reads the plate on an absorbance reader. The procedure is performed twice, once for each
Synergy HTX Operator's Manual