Flag/ Symbols
Description
Offscale
At least one data point in the analysis range has saturated
the CCD camera.
Note:
In the Monitor Run screen, an offscale sample is
flagged with
.
Spectral Pull-Up
(fragment analysis
only)
At least one peak contains a pull-up peak.
A
pull-up peak is identified when the peak height of the minor
peak is
£
X% of and within ±Y data point of the major peak,
where X and Y are values you specify.
Broad Peak (HID
analysis only)
At least one peak exceeds the Broad Peak threshold.
Broad peaks affect Sizing Quality. See Chapter 7, “Manage
Note:
The value displayed when you place the mouse pointer
over a Broad Peak flag is an internal value and does not reflect
the peak width.
Normalization
Limit
•
—Sample was collected with a normalization size
standard, sample Normalization Factor is within range.
—Sample was collected with a normalization size
standard, sample Normalization Factor is not within
range.
No Data—Normalization is enabled, but Sizing Quality is
.
NO—Sample was not collected with a normalization size
standard.
N/A—Sample was not collected on a 3500 instrument.
For more information, see “Review normalized data“ on
Note:
If the Sizing Quality is , normalization is not applied,
even if the Normalization Factor is within the normalization
range.
SQ: Sizing Quality
The Sizing Quality is in the Fail or Suspect range. Place
the mouse pointer over a flag to display the Sizing Quality
value for the sample.
3.
Click a flag in the samples table, or select samples in the samples table to display
the associated data in the plot view and sizing table view.
4.
(Optional) Modify the sample view:
• Right-click the Size Standard field to view the size standard for a sample.
• Click Minimize and Restore to collapse and expand the samples table.
Chapter 5
Review fragment/HID analysis results
Review sample quality
5
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
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