Basecalling protocols library (primary analysis—sequencing)
A basecalling protocol is the required primary analysis protocol for sequencing
applications.
A basecalling protocol defines the settings used by the sequencing basecallers to
assign base calls to each detected peak and assign a quality value:
• Analysis settings
• Ranges for the sequencing quality flags displayed in View Results
When you create a sequencing assay, you add a basecalling protocol to the assay. If
you add this item from the library, a copy of the item is added to the assay, and can be
modified independently from the original item stored in the library. For information
on how changes are tracked if auditing is enabled, see “Audit action“ on page 205.
If factory-provided basecalling protocols do not suit your needs, you can create new
basecalling protocols:
1.
Access the Basecalling Protocols library.
2.
Click
Create.
3.
In the Analysis Settings tab of the Create New Basecalling Protocol dialog box
(Figure 23), specify settings “Basecalling protocol—Analysis settings“ on
Basecalling
protocol overview
Create a new
basecalling
protocol
Chapter 7
Manage library resources
Basecalling protocols library (primary analysis—sequencing)
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