b.
Specify the order of the peaks in the calibration standard you are using. Use
the internal order number of the dye based on the dyes selected.
IMPORTANT!
The Calibration Peak Order fields do not correspond to the
dye colors displayed above the Calibration Peak Order fields.
• Example 1—If the order of the peaks in the calibration standard you are
using is Orange, Red, Yellow, Blue, Green, Purple, specify for
Calibration Peak Order: 6 (Orange), 4 (Red), 3 (Yellow), 1 (Blue), 2
(Green), 5 (Purple).
• Example 2—If the order of the peaks in the calibration standard you are
using is Orange, Red, Yellow, Blue, Green, specify for Calibration Peak
Order: 5 (Orange), 4 (Red), 3 (Yellow), 1 (Blue), 2 (Green).
• Example 3—If the order of the peaks in the calibration standard you are
using is Orange, Red, Green, specify for Calibration Peak Order: 3
(Orange), 2 (Red), 1 (Green).
6.
Expand the Parameters section, then specify remaining settings.
7.
Click Save.
Size standards library
A size standard defines the sizes of known fragments. It is used to generate a
standard curve. The standard curve is used to determine the sizing of unknown
samples.
When you create a sizecalling (fragment) or QC (HID) protocol, you add a size
standard to the protocol. If you add this item from the library, a copy of the item is
added to the protocol, and can be modified independently from the original items
stored in the library. For information on how changes are tracked if auditing is
enabled, see “Audit action“ on page 205.
Size standard
overview
Chapter 7
Manage library resources
Size standards library
7
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3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
