Setup workflow
1.
“Prepare the instrument“ on page 41
2.
“Prepare sample plates“ on page 52 and “Load the plate in the
3.
“Check system status in the Dashboard“ on page 32
q
q
1. “Create or import a plate“ on
2.
3.
4.
“Quick Start a run“ on page 58
q
q
1.
“Load plates for run and create the injection list“ on page 59
2.
“Review and modify the injection list in Preview Run“ on page 62
3.
“Start the run from Preview Run“ on page 64
4.
“Check sequence or sample quality and re-inject samples“ on
Prepare the instrument
1.
In the Dashboard, check consumables status (see “Check consumables status“ on
• Consumables are not expired
• Adequate injections remain for consumables
2.
Ensure that buffer levels are at the fill lines (see “Check buffer fill levels“ on
3.
Set the oven temperature, then click Start Pre-heat.
Pre-heat the oven and detection cell while you prepare for a run (detection cell
temperature is set by the software). Pre-heating helps mitigate subtle first-run
migration rate effects. The pre-heat function automatically turns off after 2 hours.
We recommend that you pre-heat the oven for at least 30 minutes before you
start a run if the instrument is cold.
Temperatures are displayed in red as they warm to the set-points. When
temperatures are at the set-point they are displayed in green. Temperatures may
fluctuate slightly when they reach the set-point as they stabilize.
4.
Check the pump assembly for bubbles and run the Remove Bubble wizard if
needed (see “Remove bubbles from the polymer pump“ on page 237).
Chapter 3
Set up and run
Setup workflow
3
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