Applied Biosystems 2720, User Manual

Page: 94 / 151

86 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 7 Temperature non-uniformity test
8. Slide the heated cover back, and repeat steps 4 through 7 of “Setting up the 0.5-
mL probe assembly” on page 83 and steps 2 through 7 of this procedure.
Complete these steps for all 12 wells to be tested:
A1, A6, A10, B3, C5, C10, D1, D7, E8, F1, F5, and F10
9. The GeneAmp
®
9700 evaluates the uniformity of the sample block temperature
for the setpoint values that you entered and displays the results. A summary
screen opens at the end of the test.
If you entered values on the Temperature Non-Uniformity Test Data Sheet,
compare those values with the actual test results.
10. Press F1 (Accept). To interpret the results, see “Evaluating the results” on
page 87 .
11. When you complete all measurements, be sure to:
• Press Cancel .
• Remove the 0.5-mL probe assembly from the sample block.
• Power off the digital thermometer, then clean off the oil.
• Remove the 0.5-mL temperature verification frame from the sample block.
IMPORTANT!
Make sure the sample block is at room temperature (~25 °C)
before removing the frame.
Well
F1 F3 F4 F5 F2
Accept
Cancel
94 °C 37 °C
More
xx.x A1 xx.x
xx.x A6 xx.x
xx.x A10 xx.x
xx.x B3 xx.x
Well 94 °C 37 °C
xx.x C5 xx.x
xx.x C10 xx.x
xx.x D1 xx.x
xx.x D7 xx.x