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Thermal Cycler Temperature Verification System For GeneAmp

PCR Systems 2720, 9600, 9700, 9800,

and Veriti

System User Guide

Chapter 7

Temperature non-uniformity test

Evaluating the

results

When the GeneAmp

9700 completes the temperature non-uniformity test, the TNU

Performance screen opens. See the table below to evaluate the results.

If the...

Then...

Temperature of the
sample block wells is
uniform

“Pass” appears after each setpoint temperature.

Temperature variation of
the sample block wells
exceeds performance
specifications

“Fail” appears after the setpoint temperature(s) for which the
test failed.

• If the test fails, repeat the procedure to make sure that the

thermometer was not misread or that data-entry errors were
not made.

• If the test fails again, contact Applied Biosystems Technical

Support. See

“How to obtain support” on page viii

TNU Performance

TNU at 94°C is xx.xx - Pass
TNU at 37°C is xx.xx - Pass

Cancel

TNU Performance

TNU at 94°C is xx.xx - Fail
TNU at 37°C is xx.xx - Fail

Cancel

Summary of Contents for 2720

Page 1: ...Thermal Cycler Temperature Verification System For GeneAmp PCR System 2720 9600 9700 9800 and Veriti Thermal Cyclers User Guide ...

Page 2: ...IAL INCIDENTAL INDIRECT PUNITIVE MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT INCLUDING BUT NOT LIMITED TO THE USE THEREOF NOTICE TO PURCHASER PLEASE REFER TO THE SPECIFIC GENEAMP PCR SYSTEM USER MANUAL FOR LIMITED LABEL LICENSE OR DISCLAIMER INFORMATION The Applied Biosystems Thermal Cycler Temperature Verification System is covered by U S Patent No 5 224 778...

Page 3: ...perature calibration verification test 9 Temperature non uniformity test 13 Data Sheet Calibration Verification Test 18 Data Sheet Temperature Non Uniformity Test 19 Temperature Verification Tests for the GeneAmp 9700 Thermal Cycler Overview 22 Temperature calibration verification test 23 Temperature non uniformity test 27 Data Sheet Calibration Verification Test 32 Data Sheet Temperature Non Unif...

Page 4: ...ata Sheet Calibration Verification Test 74 Data Sheet Temperature Non Uniformity Test 75 Temperature Verification Tests for the GeneAmp 9700 0 5 mL Sample Block Module Calibration verification test 78 Temperature non uniformity test 83 Data Sheet Calibration Verification Test 88 Data Sheet Temperature Non Uniformity Test 89 Temperature Verification Tests for the GeneAmp 9800 Calibration verificati...

Page 5: ...ure non uniformity test 123 Data Sheet Calibration Verification Test 127 Data Sheet Temperature Non Uniformity Test 128 Appendix A Recalibration Obtaining recalibration 129 Appendix B Instrument Warranty Information Limited product warranty 132 Damages claims and returns 134 Appendix C GeneAmp PCR 9700 Technical Note Temperature Accuracy Verification Test 135 Appendix D Safety Instrumentation safe...

Page 6: ...vi Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720 9600 9700 9800 and Veriti System User Guide ...

Page 7: ...N Indicates a potentially hazardous situation that if not avoided may result in minor or moderate injury It may also be used to alert against unsafe practices WARNING Indicates a potentially hazardous situation that if not avoided could result in death or serious injury DANGER Indicates an imminently hazardous situation that if not avoided will result in death or serious injury This signal word is...

Page 8: ... be of interest or help but is not critical to the use of the product IMPORTANT Provides information that is necessary for proper instrument operation accurate chemistry kit use or safe use of a chemical How to obtain support For the latest services and support information for all locations go to www appliedbiosystems com At the Applied Biosystems web site you can Access worldwide telephone and fa...

Page 9: ...erification System For GeneAmp PCR Systems 2720 9600 9700 9800 and Veriti System User Guide 1 Introduction 1 This chapter covers About this manual 2 About the Temperature Verification System 2 Probe assembly 5 Digital thermometer 5 ...

Page 10: ...4 About the Temperature Verification System Description The Temperature Verification System is a kit consisting of a probe a digital thermometer frames to prevent ambient air from entering the sample block light mineral oil and cotton swabs The system is used to verify temperatures of the sample block on your thermal cycler The table below indicates which Temperature Verification System kit you sh...

Page 11: ...d Veriti System User Guide Chapter 1 About this manual Figure 1 Components of the 0 2 mL 96 Well Temperature Verification Kit MODE F1 F2 4500 GR1918 Probe assembly Frame for GeneAmp 9700 9600 and 2720 thermal cycler Digital thermometer Plate for GeneAmp 9700 with dual 96 well sample block module ...

Page 12: ...lid in a court case Another laboratory may want to validate a protocol Typically the temperature calibration verification test should be performed at least once a year and the temperature non uniformity test should be run at the same time and frequency Running these tests verifies that the thermal cycler is operating to Applied Biosystems operating specifications Kit parts The kit is supplied with...

Page 13: ...es the temperature of the sample well The temperature is measured by a thermistor bead in the cone tip Digital thermometer Overview The handheld digital thermometer shown below has a temperature range of 0 to 100 C and is accurate to within 0 1 C IMPORTANT Do not try to recalibrate or perform any service on the digital thermometer The only user serviceable component in the unit is the battery GR19...

Page 14: ...ffers from the temperature that is displayed on the digital thermometer during heating or cooling transitions This is because the thermal cycler displays the calculated sample temperature while the digital thermometer measures and displays the sample block temperature The sample temperature that is displayed by the thermal cycler is calculated based on the ramp rate and reaction volume Using the p...

Page 15: ...0 and Veriti System User Guide 2 Temperature Verification Tests for the 2720 Thermal Cycler 2 This chapter covers Overview 8 Temperature calibration verification test 9 Temperature non uniformity test 13 Data Sheet Calibration Verification Test 18 Data Sheet Temperature Non Uniformity Test 19 ...

Page 16: ... Assembly Figure 3 shows a properly assembled system Note that the frame is positioned around the wells with the channel facing you The location tab on the frame should align with the locator tab on the thermal cycler The probe wire is threaded through the channel to prevent damaging the wire when you close the thermal cycler heated cover Figure 3 Proper assembly of the Temperature Verification Sy...

Page 17: ...tal thermometer with a 9V battery installed Setting up the 0 2 mL probe assembly 1 Lift the lever and open the hinged heated cover 2 Place the 2720 temperature verification frame on the sample block 3 Using a cotton swab coat well A6 with mineral oil 4 Place the 0 2 mL probe assembly into well A6 5 Thread the probe wire through the channel in the 2720 temperature verification frame to prevent dama...

Page 18: ...run the calibration verification test 1 Press F1 Run to start the calibration verification test The Calibration Verification screen opens with the setpoint value displayed Note The cover must be at 85 C 10 C It may take several minutes for the system to ramp up Note The Calibration Verification screen counts down the time until the setpoint is reached Temperature Verification Temp Calibration Veri...

Page 19: ...cycler automatically begins the second temperature reading 45 C setpoint The Calibration Verification screen displays the setpoint value Note The cover must be at 45 C 30 C It may take several minutes for the system to ramp up 5 Repeat steps 2 through 4 for the second temperature reading The thermal cycler evaluates the calibration of the sample block temperature for the setpoint values that you e...

Page 20: ...lock 3 Power off the digital thermometer then clean off the oil Wait for the sample block to reach room temperature 25 C then remove the 2720 temperature verification frame from the sample block PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components 4 Clean the oil off the sample block If the sample block module The...

Page 21: ...frame 0 2 mL probe assembly Model 4500 digital thermometer with a 9V battery installed Setting up the 0 2 mL probe assembly 1 Lift the lever and open the hinged heated cover 2 Place the 2720 temperature verification frame on the sample block 3 Use a cotton swab to coat the following wells with mineral oil A1 A12 C4 C9 F4 F9 H1 H12 4 Place the 0 2 mL probe assembly into well A1 then as the test pro...

Page 22: ...s After the system is configured the TNU Performance screen opens Running the test The temperature non uniformity test uses the 0 2 mL probe assembly to test the temperature uniformity of 8 different wells in the sample block Note Press F5 Cancel if you want to exit the test To run the temperature non uniformity test 1 Place the probe into the indicated well then press F1 Run The temperature non u...

Page 23: ...e value to three digits before you enter it in the TNU Performance screen Record the value on the Temperature Non Uniformity Test Data Sheet page 19 to keep a permanent record of the test 5 Press Enter The following TNU Performance screen opens and the system automatically begins decreasing its setpoint temperature to 37 C 6 When the setpoint temperature reaches 37 C the TNU Performance screen ope...

Page 24: ...l cycler completes the temperature non uniformity test the TNU Performance screen opens See the table below to evaluate the results Well F1 F3 F4 F5 F2 Accept Cancel xx x A1 xx x xx x A12 xx x xx x C4 xx x xx x C9 xx x Well xx x F4 xx x xx x F9 xx x xx x H1 xx x xx x H12 xx x 37 C 94 C 37 C 94 C If the Then Temperature of the sample block wells is uniform Pass appears after each setpoint temperatu...

Page 25: ...e 0 2 mL probe assembly from the sample block 3 Power off the digital thermometer then clean off the oil 4 Wait for the sample block to reach room temperature 25 C then remove the 2720 temperature verification frame from the sample block PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components 5 Clean the oil off the ...

Page 26: ...Sheet Calibration Verification Test When you run the calibration verification test record the setpoint values for well A6 on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value Well A6 85 C 45 C ...

Page 27: ...on Uniformity Test When you run the temperature non uniformity test record the setpoint values for the wells that are listed on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value 94 C 37 C A1 A12 C4 C9 F4 F9 H1 H...

Page 28: ...20 Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720 9600 9700 9800 and Veriti System User Guide Chapter 2 Data Sheet Temperature Non Uniformity Test ...

Page 29: ... Veriti System User Guide 3 Temperature Verification Tests for the GeneAmp 9700 Thermal Cycler 3 This chapter covers Overview 22 Temperature calibration verification test 23 Temperature non uniformity test 27 Data Sheet Calibration Verification Test 32 Data Sheet Temperature Non Uniformity Test 33 ...

Page 30: ...PN 4317939 Assembly Figure 5 shows a properly assembled system Note that the frame is positioned around the wells with the channel facing you The location tab on the frame should align with the locator tab on the thermal cycler The probe wire is threaded through the channel to prevent damaging the wire when you close the thermal cycler heated cover Figure 5 Proper assembly of the Temperature Verif...

Page 31: ...a 9V battery installed Setting up the 0 2 mL probe assembly 1 If the heated cover is in the forward position lift the lever then slide the heated cover back 2 Place the 9700 temperature verification frame on the sample block 3 Using a cotton swab coat well A6 with mineral oil 4 Place the 0 2 mL probe assembly into well A6 5 Thread the probe wire through the channel in the 9700 temperature verifica...

Page 32: ...o run the calibration verification test 1 Press F1 Run to start the calibration verification test The Calibration Verification screen opens with the setpoint value displayed Note The cover must be at 85 C 10 C It may take several minutes for the system to ramp up The Calibration Verification screen counts down the time until the setpoint is reached Temperature Verification Temp Calibration Verific...

Page 33: ...cycler automatically begins the second temperature reading 45 C setpoint The Calibration Verification screen displays the setpoint value Note The cover must be at 45 C 30 C It may take several minutes for the system to ramp up 5 Repeat steps 2 through 4 for the second temperature reading The thermal cycler evaluates the calibration of the sample block temperature for the setpoint values that you e...

Page 34: ... block 3 Power off the digital thermometer then clean off the oil Wait for the sample block to reach room temperature 25 C then remove the 9700 temperature verification frame from the sample block PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components 4 Clean the oil off the sample block If the sample block module T...

Page 35: ...sembly Model 4500 digital thermometer with a 9V battery installed Setting up the 0 2 mL probe assembly 1 If the heated cover is in the forward position lift the lever then slide the heated cover back 2 Place the 9700 temperature verification frame on the sample block 3 Use a cotton swab to coat the following wells with mineral oil A1 A12 C4 C9 F4 F9 H1 H12 4 Place the 0 2 mL probe assembly into we...

Page 36: ... the system is configured the TNU Performance screen opens Running the test The temperature non uniformity test uses the 0 2 mL probe assembly to test the temperature uniformity of 8 different wells in the sample block Note Press F5 Cancel if you want to exit the test To run the temperature non uniformity test 1 Place the probe into the indicated well then press F1 Run The temperature non uniformi...

Page 37: ...e value to three digits before you enter it in the TNU Performance screen Record the value on the Temperature Non Uniformity Test Data Sheet page 33 to keep a permanent record of the test 5 Press Enter The following TNU Performance screen opens and the system automatically begins decreasing its setpoint temperature to 37 C 6 When the setpoint temperature reaches 37 C the TNU Performance screen ope...

Page 38: ...ermal cycler completes the temperature non uniformity test the TNU Performance screen opens See the table below to evaluate the results Well F1 F3 F4 F5 F2 Accept Cancel xx x C4 xx x xx x C9 xx x xx x F4 xx x xx x F9 xx x Well xx x A1 xx x xx x A12 xx x xx x H1 xx x xx x H12 xx x 37 C 94 C 37 C 94 C If the Then Temperature of the sample block wells is uniform Pass appears after each setpoint tempe...

Page 39: ...e 0 2 mL probe assembly from the sample block 3 Power off the digital thermometer then clean off the oil 4 Wait for the sample block to reach room temperature 25 C then remove the 9700 temperature verification frame from the sample block PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components 5 Clean the oil off the ...

Page 40: ...Sheet Calibration Verification Test When you run the calibration verification test record the setpoint values for well A6 on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value Well A6 85 C 45 C ...

Page 41: ...on Uniformity Test When you run the temperature non uniformity test record the setpoint values for the wells that are listed on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value 94 C 37 C C4 C9 F4 F9 A1 A12 H1 H...

Page 42: ...34 Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720 9600 9700 9800 and Veriti System User Guide Chapter 3 Data Sheet Temperature Non Uniformity Test ...

Page 43: ... and Veriti System User Guide 4 Temperature Verification Tests for the GeneAmp 9700 Dual 96 Well Module 4 This chapter covers Overview 36 Calibration verification test 37 Temperature non uniformity test 42 Data Sheet Calibration Verification Test 47 Data Sheet Temperature Non Uniformity Test 48 ...

Page 44: ...ample block module you use the materials in the kit PN 4317939 Assembly Figure 7 show the right frame of a properly assembled temperature verification system Note that the probe wire is threaded through the channel to prevent damage to the wire when you close the thermal cycler heated cover Figure 7 The temperature verification system for GeneAmp 9700 with a dual 96 well sample block module right ...

Page 45: ...mL probe assembly Model 4500 digital thermometer with a 9V battery installed Setting up the 0 2 mL probe assembly 1 For GeneAmp 9700 with Manual Dual 96 well Sample Block Module If the heated cover is in the forward position lift the lever then slide the heated cover back For GeneAmp 9700 with Auto Lid dual 96 Well Sample Block Module If the heated cover is in the forward position lift the lever t...

Page 46: ... in your Temperature Verification Kit for details on operating the digital thermometer Model 4500 Configuring the GeneAmp 9700 1 Power on the thermal cycler The Main menu opens For best results allow the thermal cycler 15 to 30 minutes to warm up to ambient temperature before performing the test 2 Press F4 Util The Utilities screen opens 3 Press F1 Diag The Diagnostics screen opens 4 Press F3 TmpV...

Page 47: ...s to 0 read the digital thermometer Refer to the instructions in your Temperature Verification Kit for details on operating the digital thermometer Model 4500 3 Using the numeric keys enter the value that is displayed on the digital thermometer in the Enter actual block temperature field Note The digital thermometer displays a four digit value round this off to three digits before typing it in the...

Page 48: ... 2 through 4 for the second temperature reading The GeneAmp 9700 evaluates the calibration of the sample block temperature for the setpoint values that you entered and displays the results A summary screen opens at the end of the test 6 If you entered values on the Calibration Verification Test Data Sheet page 47 compare those values with the actual test results 7 Press F1 Accept Calibration Verif...

Page 49: ...INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components 5 Clean the oil off the sample block If the sample block module Then the Is properly calibrated Calibration Verification screen displays the following message Does not pass the calibration verification test Calibration Verification screen displays the following message I...

Page 50: ...ed Setting up the 0 2 mL probe assembly 1 For the GeneAmp 9700 with Manual Dual 96 Well Sample Block Module If the heated cover is in the forward position lift the lever then slide the heated cover back For the GeneAmp 9700 with Auto Lid Dual 96 Well Sample Block Module If the heated cover is in the forward position lift the lever then press the red button to slide the heated cover back 2 Place bo...

Page 51: ...it for details on operating the digital thermometer Model 4500 Configuring the GeneAmp 9700 1 Power on the thermal cycler The Main menu opens For best results allow the thermal cycler 15 to 30 minutes to warm up to ambient temperature before performing the test 2 Press F4 Util The Utilities screen opens 3 Press F1 Diag The Diagnostics screen opens 4 Press F3 TmpVer The Temperature Verification scr...

Page 52: ... time until the setpoint is stable 2 When the Stabilizing at setpoint value decreases to 0 read the digital thermometer Refer to the instructions in your Temperature Verification Kit for details on operating the digital thermometer Model 4500 3 Using the numeric keys enter the value that is displayed on the digital thermometer in the Enter actual block temperature field Note The digital thermomete...

Page 53: ...e Press the red button to slide and close the heated cover 8 Repeat steps 4 through 7 of Setting up the 0 2 mL probe assembly on page 42 and steps 1 through 7 of this procedure Complete these steps for all the remaining 11 wells to be tested L2 to L6 and R1 to R6 The GeneAmp 9700 evaluates the uniformity of the sample block temperature for the setpoint values that you entered and displays the resu...

Page 54: ...URY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components 5 Clean the oil off the sample block If the Then Temperature of the sample block wells is uniform Pass appears after each setpoint temperature Temperature variation of the sample block wells exceeds performance specifications Fail appears after the setpoint temperature s fo...

Page 55: ...Sheet Calibration Verification Test When you run the calibration verification test record the setpoint values for well R3 on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value Well R3 85 C 45 C ...

Page 56: ...y Test When you run the temperature non uniformity test record the setpoint values for the wells L1 to L6 and R1 to R6 listed on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial No Setpoint value 95 C 55 C L1 L2 L3 L4 L5 L6 R1 R...

Page 57: ...and Veriti System User Guide 5 Temperature Verification Tests for the GeneAmp 9700 Dual 384 Well Sample Block Module 5 This chapter covers Calibration verification test 50 Temperature non uniformity test 55 Data Sheet Calibration Verification Test 60 Data Sheet Temperature Non Uniformity Test 61 ...

Page 58: ...rification plates 0 02 mL probe assembly Digital thermometer Model 4500 with a 9V battery installed Setting up the 0 02 mL probe assembly 1 If the heated cover is in the forward position lift the lever then slide the heated cover back 2 Place both 384 well temperature verification plates on the sample blocks IMPORTANT The temperature verification plates must be installed in both sample blocks thro...

Page 59: ...ures in Setting up the 0 02 mL probe assembly above 2 Power on the thermal cycler The Main menu opens For best results allow the thermal cycler 15 to 30 minutes to warm up to ambient temperature before performing the test 3 Press F4 Util The Utilities screen opens 4 Press F1 Diag The Diagnostics screen opens 5 Press F3 TmpVer The Temperature Verification screen opens 6 Press F1 Temp to automatical...

Page 60: ...meter Refer to the instructions in your Temperature Verification Kit for details on operating the digital thermometer Model 4500 4 Using the numeric keys enter the value that is displayed on the digital thermometer in the Enter actual block temperature field Note Round off the four digit value on the digital thermometer to three digits before you enter it into the Calibration Verification screen N...

Page 61: ...tion Verification Test Data Sheet compare those values with the actual test results 8 Press F1 Accept To interpret the results see Evaluating the results on page 54 9 When you complete all measurements be sure to Press Exit Remove the 0 02 mL probe assembly from the sample blocks Power off the digital thermometer then clean off the oil Remove the 384 well temperature verification plates from the s...

Page 62: ...tion Verification screen displays the following message Does not pass the calibration verification test Calibration Verification screen displays the following message If the test fails repeat the procedure to make sure that the thermometer was not misread or that data entry errors were not made If the test fails again contact Applied Biosystems Technical Support See How to obtain support on page v...

Page 63: ...led Setting up the 0 02 mL probe assembly 1 If the heated cover is in the forward position lift the lever then slide the heated cover back 2 Place both 384 well temperature verification plates on the sample blocks IMPORTANT The temperature verification plates must be installed in both sample blocks throughout all temperature verification testing The plate labeled L1 to L6 is for the left sample bl...

Page 64: ... digital thermometer Model 4500 9 Continue with Configuring the GeneAmp 9700 below Configuring the GeneAmp 9700 1 Complete the procedures in Setting up the 0 02 mL probe assembly above 2 Power on the thermal cycler The Main menu opens For best results allow the thermal cycler 15 to 30 minutes to warm up to ambient temperature before performing the test 3 Press F4 Util The Utilities screen opens 4 ...

Page 65: ... Performance screen counts down the time until the setpoint is stable When the Stabilizing at setpoint value decreases to 0 read the digital thermometer Refer to the instructions in your Temperature Verification Kit for details on operating the digital thermometer Model 4500 4 Using the numeric keys enter the value that is displayed on the digital thermometer in the Enter actual block temperature ...

Page 66: ...ested 9 The GeneAmp 9700 evaluates the uniformity of the sample block temperature for the setpoint values that you entered and displays the results A summary screen opens at the end of the test If you entered values on the Temperature Non Uniformity Test Data Sheet compare those values with the actual test results 10 Press F1 Accept To interpret the results see Evaluating the results on page 59 Le...

Page 67: ...perature non uniformity test the TNU Performance screen opens See the table below to evaluate the results If the Then Temperature of the sample block wells is uniform Pass appears after each setpoint temperature Temperature variation of the sample block wells exceeds performance specifications Fail appears after the setpoint temperature s for which the test failed If the test fails repeat the proc...

Page 68: ...Sheet Calibration Verification Test When running the calibration verification test record the setpoint values for well R3 on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value Well R3 85 C 45 C ...

Page 69: ...n Uniformity Test When running the temperature non uniformity test record the setpoint values for the wells listed on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value 94 C 37 C L1 L2 L3 L4 L5 L6 R1 R2 R3 R4 R5 ...

Page 70: ...62 Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720 9600 9700 9800 and Veriti System User Guide Chapter 5 Data Sheet Temperature Non Uniformity Test ...

Page 71: ...0 and Veriti System User Guide 6 Temperature Verification Tests for the GeneAmp 9700 Auto Lid Sample Block Module 6 This chapter covers Calibration verification test 64 Temperature non uniformity test 69 Data Sheet Calibration Verification Test 74 Data Sheet Temperature Non Uniformity Test 75 ...

Page 72: ...n Kit PN 4308354 which includes Light mineral oil Two 384 well temperature verification plates 20 µL probe assembly Digital thermometer with a 9V battery installed Note This kit is also used for the Auto Lid sample block module Setting up the 20 µL probe assembly 1 Make sure the heated cover is in the rear position If not press and release the red button to automatically move the heated cover to t...

Page 73: ...be may be damaged 8 Power on the digital thermometer Refer to the instructions in your Temperature Verification Kit for details on operating the digital thermometer Model 4500 Configuring the GeneAmp 9700 1 Power on the thermal cycler The Main menu opens For best results allow the thermal cycler 15 to 30 minutes to warm up to ambient temperature before performing the test 2 Press F4 Util The Utili...

Page 74: ...int is reached b When the Stabilizing at setpoint value decreases to 0 read the digital thermometer Refer to the instructions in your Temperature Verification Kit for details on operating the digital thermometer Model 4500 3 Using the numeric keys enter the value that is displayed on the digital thermometer in the Enter actual block temperature field Note The digital thermometer displays a four di...

Page 75: ...If you entered values on the Calibration Verification Test Data Sheet compare those values with the actual test results 7 Press F1 Accept To interpret the results see Evaluating the results on page 68 8 When you complete all measurements be sure to Press F5 Exit Remove the 20 µL probe assembly from the sample blocks Power off the digital thermometer then clean off the oil Remove the 384 well tempe...

Page 76: ...tion Verification screen displays the following message Does not pass the calibration verification test Calibration Verification screen displays the following message If the test fails repeat the procedure to make sure that the thermometer was not misread or that data entry errors were not made If the test fails again contact Applied Biosystems Technical Support See How to obtain support on page v...

Page 77: ...t PN 4308354 which includes Light mineral oil Two 384 well temperature verification plates 20 µL probe assembly Digital thermometer with a 9V battery installed Note This kit is also used for the Auto Lid sample block module Setting up the 20 µL probe assembly 1 Make sure the heated cover is in the rear position If not press and release the red button to automatically move the heated cover to the r...

Page 78: ...gital thermometer Refer to the instructions in your Temperature Verification Kit for details on operating the digital thermometer Model 4500 Configuring the GeneAmp 9700 1 Power on the thermal cycler The Main menu opens For best results allow the thermal cycler 15 to 30 minutes to warm up to ambient temperature before performing the test 2 Press F4 Util The Utilities screen opens 3 Press F1 Diag T...

Page 79: ...the digital thermometer Refer to the instructions in your Temperature Verification Kit for details on operating the digital thermometer Model 4500 3 Using the numeric keys enter the value that is displayed on the digital thermometer in the Enter actual block temperature field Note The digital thermometer displays a four digit value round this off to three digits before typing it in the TNU Perform...

Page 80: ...for all 12 wells to be tested 8 The GeneAmp 9700 evaluates the uniformity of the sample block temperature for the setpoint values that you entered and displays the results A summary screen opens at the end of the test If you entered values on the Temperature Non Uniformity Test Data Sheet compare those values with the actual test results 9 Press F1 Accept To interpret the results see Evaluating th...

Page 81: ...perature non uniformity test the TNU Performance screen opens See the table below to evaluate the results If the Then Temperature of the sample block wells is uniform Pass appears after each setpoint temperature Temperature variation of the sample block wells exceeds performance specifications Fail appears after the setpoint temperature s for which the test failed If the test fails repeat the proc...

Page 82: ...Sheet Calibration Verification Test When running the calibration verification test record the setpoint values for well R3 on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value Well R3 85 C 45 C ...

Page 83: ...n Uniformity Test When running the temperature non uniformity test record the setpoint values for the wells listed on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value 94 C 37 C L1 L2 L3 L4 L5 L6 R1 R2 R3 R4 R5 ...

Page 84: ...76 Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720 9600 9700 9800 and Veriti System User Guide Chapter 6 Data Sheet Temperature Non Uniformity Test ...

Page 85: ...00 and Veriti System User Guide 7 Temperature Verification Tests for the GeneAmp 9700 0 5 mL Sample Block Module 7 This chapter covers Calibration verification test 78 Temperature non uniformity test 83 Data Sheet Calibration Verification Test 88 Data Sheet Temperature Non Uniformity Test 89 ...

Page 86: ...hermometer Model 4500 with a 9V battery installed Setting up the 0 5 mL probe assembly 1 If the heated cover is in the forward position lift the lever then slide the heated cover back 2 Place the GeneAmp 0 5 mL temperature verification frame on the sample block 3 Use a cotton swab to coat well A6 with mineral oil 4 Place the 0 5 mL probe assembly into well A6 5 Thread the probe wire through the ch...

Page 87: ...emperature before performing the test 3 Press F4 Util The Utilities screen opens 4 Press F1 Diag The Diagnostics screen opens 5 Press F3 TmpVer The Temperature Verification screen opens 6 Press F1 Temp to automatically configure the GeneAmp 9700 for the calibration verification test The Calibration Verification screen opens 7 Continue with Running the test on page 80 Temperature Verification Temp ...

Page 88: ...ts down the time until the setpoint is reached When the Stabilizing at setpoint value decreases to 0 read the digital thermometer Refer to the instructions in your Temperature Verification Kit for details on operating the digital thermometer Model 4500 4 Using the numeric keys enter the value that is displayed on the digital thermometer in the Enter actual block temperature field Note The digital ...

Page 89: ...bration Verification Test Data Sheet compare those values with the actual test results 8 Press F1 Accept To interpret the results see Evaluating the results on page 82 9 When you complete all measurements be sure to Press Exit Remove the 0 5 mL probe assembly from the sample block Power off the digital thermometer then clean off the oil Remove the 0 5 mL temperature verification frame from the sam...

Page 90: ...tion Verification screen displays the following message Does not pass the calibration verification test Calibration Verification screen displays the following message If the test fails repeat the procedure to make sure that the thermometer was not misread or that data entry errors were not made If the test fails again contact Applied Biosystems Technical Support See How to obtain support on page v...

Page 91: ...ion frame on the sample block 3 Use a cotton swab to coat the following wells with mineral oil A1 A6 A10 B3 C5 C10 D1 D7 E8 F1 F5 F10 4 Place the 0 5 mL probe assembly into well A1 Note As the test progresses you move the 0 5 mL probe assembly to each of the test wells 5 Thread the probe wire through the channel in the 0 5 mL temperature verification frame to prevent damage to the probe and lead w...

Page 92: ... sample block Note If necessary press F5 Cancel to exit the test To run the temperature non uniformity test 1 Complete the procedures in Setting up the 0 5 mL probe assembly above 2 Press F1 Run to start the temperature non uniformity test The TNU Performance screen opens with the setpoint value displayed Note The sample block must be within 1 0 C of the setpoint In addition the cover must be with...

Page 93: ...een Note Record this value on the Temperature Non Uniformity Test Data Sheet page 89 to keep a permanent record of the test 5 Press ENTER The GeneAmp 9700 automatically begins the second reading 37 C setpoint The TNU Performance screen opens with the setpoint value displayed Note The sample block must be within 1 0 C of the setpoint 6 Repeat steps 3 and 4 for the second reading 7 Press ENTER The T...

Page 94: ... summary screen opens at the end of the test If you entered values on the Temperature Non Uniformity Test Data Sheet compare those values with the actual test results 10 Press F1 Accept To interpret the results see Evaluating the results on page 87 11 When you complete all measurements be sure to Press Cancel Remove the 0 5 mL probe assembly from the sample block Power off the digital thermometer ...

Page 95: ...rs after each setpoint temperature Temperature variation of the sample block wells exceeds performance specifications Fail appears after the setpoint temperature s for which the test failed If the test fails repeat the procedure to make sure that the thermometer was not misread or that data entry errors were not made If the test fails again contact Applied Biosystems Technical Support See How to o...

Page 96: ...Sheet Calibration Verification Test When running the calibration verification test record the setpoint values for well A6 on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value Well A6 85 C 45 C ...

Page 97: ... Uniformity Test When running the temperature non uniformity test record the setpoint values for the wells listed on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value 94 C 37 C A1 A6 A10 B3 C5 C10 D1 D7 E8 F1 F5...

Page 98: ...90 Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720 9600 9700 9800 and Veriti System User Guide Chapter 7 Data Sheet Temperature Non Uniformity Test ...

Page 99: ... 9600 9700 9800 and Veriti System User Guide 8 Temperature Verification Tests for the GeneAmp 9800 8 This chapter covers Calibration verification test 92 Temperature non uniformity test 97 Data Sheet Calibration Verification Test 103 Data Sheet Temperature Non Uniformity Test 104 ...

Page 100: ... cover is in the forward position lift the lever then slide the heated cover back 2 With the instrument powered off and the sample block at room temperature place the 0 1 mL temperature verification probe assembly on the sample blocks Make sure the undercut of the 0 1 mL temperature verification probe assembly is facing outwards 3 Use a cotton swab to coat well A6 with mineral oil 4 Place the 0 1 ...

Page 101: ...cation screen 6 Press F1 Temp This action opens the Calibration Verification screen and configures the GeneAmp 9800 for the calibration verification test 7 Continue with Running the test below Running the test Use the digital thermometer to take temperature readings of the sample well that is connected to the 0 1 mL probe assembly You take a reading at each of two setpoint temperatures Note If nec...

Page 102: ...ation Kit for details on operating the digital thermometer Model 4500 4 Using the numeric keys enter the value that is displayed on the digital thermometer into the Enter actual block temperature field Note Round the four digit value on the digital thermometer to three digits before you enter it into the Calibration Verification screen Note You can record this value on the Calibration Verification...

Page 103: ...fication Test Data Sheet compare those values with the actual test results 7 Press F1 Accept To interpret the results see Evaluating the results on page 96 8 When you complete all measurements be sure to Press Exit Remove the 0 1 mL probe assembly from the sample blocks Power off the digital thermometer then clean off the oil Remove the 0 1 mL temperature verification probe assembly from the sampl...

Page 104: ...tion Verification screen displays the following message Does not pass the calibration verification test Calibration Verification screen displays the following message If the test fails repeat the procedure to make sure that the thermometer was not misread or that data entry errors were not made If the test fails again contact Applied Biosystems Technical Support See How to obtain support on page v...

Page 105: ...in the forward position lift the lever then slide the heated cover back 2 With the instrument powered off and the sample block at room temperature place the 0 1 mL temperature verification probe assembly on the sample block 3 Use a cotton swab to coat wells A1 A12 C4 C9 F4 F9 H1 and H12 with mineral oil 4 Place the 0 1 mL probe assembly into well A1 As the test progresses move the 0 1 mL probe ass...

Page 106: ... the Diagnostics screen 5 Press F3 TmpVer to open the Temperature Verification screen 6 Press F2 TNU to open the TNU Performance screen and configure the 9800 for the temperature non uniformity test Note If the cover temperature is not within 1 C of the heated cover setpoint the following screen opens 7 Continue with Running the test on page 99 Temperature Verification Temp Calibration Verificatio...

Page 107: ...he time until the setpoint is stable Note The sample blocks must be within 1 0 C of the setpoint In addition the cover must be within 1 C of 55 C It may take several minutes for the GeneAmp 9800 to ramp up 3 When the TNU Performance screen opens to display the setpoint temperature press F5 Cancel 4 When the Stabilizing at setpoint value decreases to 0 read the digital thermometer Refer to the inst...

Page 108: ...e Temperature Non Uniformity Test Data Sheet page 104 to keep a permanent record of the test 6 Press Enter to open the TNU Performance screen with the setpoint value displayed Note The sample blocks must be within 1 0 C of the setpoint 7 Press Enter The TNU Performance screen opens with the following prompt TNU Performance Sample temp xx x C Cover temp xxx C F1 F3 F4 F5 F2 Cancel Enter actual bloc...

Page 109: ... F4 F9 H1 and H12 10 When the TNU Performance screen opens to display the setpoint temperature press F5 Cancel The GeneAmp 9800 evaluates the uniformity of the sample block temperature for the setpoint values that you entered and displays the results A summary screen opens at the end of the test If you entered values on the Temperature Non Uniformity Test Data Sheet compare those values with the a...

Page 110: ...rformance screen opens Use the table below to evaluate the results If the Then Temperature of the sample block wells is uniform Pass appears after each setpoint temperature Temperature variation of the sample block wells exceeds performance specifications Fail appears after the setpoint temperature s for which the test failed If the test fails repeat the procedure to make sure that the thermometer...

Page 111: ...Sheet Calibration Verification Test When running the calibration verification test record the setpoint values for well A6 on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value Well A6 85 C 45 C ...

Page 112: ...iformity Test When running the temperature non uniformity test record the setpoint values for wells A1 A12 C4 C9 F4 F9 H1 and H12 on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value 95 C 55 C A1 A12 C4 C9 F4 F9...

Page 113: ...700 9800 and Veriti System User Guide 9 Temperature Verification Tests for the GeneAmp 9600 9 This chapter covers Overview 106 Calibration verification test 107 Temperature non uniformity test 111 Data Sheet Calibration Verification Test 114 Data Sheet Temperature Non Uniformity Test 115 ...

Page 114: ...fication tests on the GeneAmp 9600 you use the materials provided in your kit Assembly Figure 18 shows a properly assembled system The frame is positioned around the wells with the channel facing you and the probe wire is threaded through the channel to prevent damaging the wire when the thermal cycler heated cover is closed Figure 18 The Temperature Verification System for GeneAmp 9600 GR1916 ...

Page 115: ...ometer with a 9V battery installed Setting up the 0 2 mL probe assembly 1 If the heated cover is in the forward position turn the knob counterclockwise completely then slide the heated cover back 2 Place the 9600 temperature verification frame on the sample block 3 Using a cotton swab coat well E1 with mineral oil 4 Place the 0 2 mL probe assembly into well E1 5 Thread the probe wire through the c...

Page 116: ...le block are the same in this test This condition prevents the heated cover from affecting the accuracy of the probe assembly The temperature of the sample block and heated cover increases to 40 C and the following display opens This display shows the current temperature of the block cover Cvr xxC and sample block Blk xx xC When the temperature of the block cover is within 10 C of the sample block...

Page 117: ...e following display opens 7 Measure the temperature of well E1 using the digital thermometer Record this temperature as T 95 IMPORTANT To end the test at any time press the STOP key This returns you to the Review History File display Press 5 then press ENTER to return to the Verify Calibration Diagnostic test The figure below shows temperatures during the calibration verification test Calculating ...

Page 118: ...lock when the instrument was calibrated at the factory Average block temperature at the 40 C hold Use the following formula to calculate the average block temperature at the 40 C hold Block Average at 40 C T 40 Low Offset If the block average is more than 0 75 degrees Celsius above or below 40 C your GeneAmp 9600 must be recalibrated For example If the measured temperature of well E1 is 39 9 C and...

Page 119: ...tely counterclockwise then slide the heated cover back 2 Using a cotton swab coat the following wells with mineral oil A1 A4 A8 A12 C1 C4 C8 C12 E1 E4 E8 E12 H1 H4 H8 H12 3 Place the 9600 temperature verification frame on the sample block with the channel facing the front of the instrument 4 Place the 0 2 mL probe assembly into well A1 5 Thread the probe wire through the channel in the 9600 temper...

Page 120: ...ck on the run time display reads 0 30 30 seconds Record this temperature The figure below shows when to measure the temperatures 5 After you measure the second temperature of well A1 turn the cover knob completely counterclockwise then slide the heater cover back 6 Move the probe assembly to the next well to be measured 7 Slide the heater cover forward then turn the cover knob clockwise until the ...

Page 121: ... the highest measured temperature For the 16 Setpoint 2 measurements 40 C hold subtract the lowest measured temperature from the highest measured temperature If either result is more than 1 C from the setpoint your GeneAmp 9600 must be serviced by an Applied Biosystems service representative Ending the test 1 Remove the 0 2 mL probe assembly from the sample block 2 Power off the digital thermomete...

Page 122: ...Sheet Calibration Verification Test When you run the calibration verification test record the setpoint values for well E1 on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value Well E1 95 C 40 C ...

Page 123: ...rmity Test When you run the temperature non uniformity test record the setpoint values for the wells listed on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Setpoint value 95 C 40 C A1 A4 A8 A12 C1 C4 C8 C12 E1 E4 E8 E12 H...

Page 124: ...116 Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720 9600 9700 9800 and Veriti System User Guide Chapter 9 Data Sheet Temperature Non Uniformity Test ...

Page 125: ...00 and Veriti System User Guide 10 Temperature Verification Tests for the Veriti Thermal Cycler 10 This chapter covers Overview 118 Calibration verification test 119 Temperature non uniformity test 123 Data Sheet Calibration Verification Test 127 Data Sheet Temperature Non Uniformity Test 128 ...

Page 126: ...ion tests on the Veriti Thermal Cycler you use the materials provided in your kit Assembly Figure 19 shows a properly assembled system The frame is positioned around the wells with the channel facing you and the probe wire is threaded through the channel to prevent damaging the wire when the thermal cycler heated cover is closed Figure 19 The Temperature Verification System for Veriti Thermal Cycl...

Page 127: ...it PN 4309924 which includes Cotton swabs Light mineral oil Temperature verification frame 0 5 mL probe assembly Model 4500 digital thermometer with a 9V battery installed Serial Communication Cable Setting up the 0 5 mL probe assembly 1 Power on the Veriti Thermal Cycler For best results allow the thermal cycler 15 to 30 minutes to warm to ambient temperature before performing the test 2 In the M...

Page 128: ... in the temperature verification frame to prevent damage to the probe assembly and lead wires see Figure 19 on page 118 IMPORTANT Seat the probe properly and the close the heated cover carefully If the probe wire is crushed when you close the heated cover the probe can be damaged 8 Power on the digital thermometer Refer to the instructions in your Temperature Verification Kit for details on operat...

Page 129: ... test 1 In the Tools Menu select Run Temperature Verification 2 Press to begin the test Perform the test as directed by the touchscreen As the test progresses move the 0 5 mL probe assembly to the remaining test wells B8 F1 F10 3 When the test is complete record zonal temperatures in the Data Sheet Calibration Verification Test on page 127 4 Press to return to the Tools menu ...

Page 130: ...eated cover and sample block to avoid being burned by hot components 2 Wait for the sample block to reach room temperature 25 C then remove the the 0 5 mL probe assembly and thermal isolation frame from the sample block 3 If you are performing a repeat measurement clean the probe assembly and all wells of mineral oil then perform the repeat measurement Otherwise power off the Veriti Thermal Cycler...

Page 131: ... Kit PN 4309924 which includes Cotton swabs Light mineral oil Temperature verification frame 0 5 mL probe assembly Model 4500 digital thermometer with a 9V battery installed Serial Communication Cable Setting up the 0 5 mL probe assembly 1 Power on the Veriti Thermal Cycler For best results allow the thermal cycler 15 to 30 minutes to warm to ambient temperature before performing the test 2 In the...

Page 132: ...nel in the temperature verification frame to prevent damage to the probe assembly and lead wires see Figure 19 on page 118 IMPORTANT Seat the probe properly and the close the heated cover carefully If the probe wire is crushed when you close the heated cover the probe can be damaged 8 Power on the digital thermometer Refer to the instructions in your Temperature Verification Kit for details on ope...

Page 133: ...ng the test 1 In the Tools menu select Run TNU Test 2 Press to begin the test Perform the test as directed by the touchscreen As the test progresses move the 0 5 mL probe assembly to the remaining test wells A1 A10 B3 B8 E3 E8 F1 F10 3 When the test is complete record the TNU in the Data Sheet Temperature Non Uniformity Test on page 128 4 Press to return to the Tools menu ...

Page 134: ...heated cover and sample block to avoid being burned by hot components 2 Wait for the sample block to reach room temperature 25 C then remove the the 0 5 mL probe assembly and thermal isolation frame from the sample block 3 If you are performing a repeat measurement clean the probe assembly and all wells of mineral oil then perform the repeat measurement Otherwise power off the Veriti Thermal Cycle...

Page 135: ...n Test When you run the calibration verification test record the setpoint values for well E1 on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Avg Probe Temp 1 at 85 C Avg Probe Temp 2 at 45 C Left Zone Right Zone Left Zone...

Page 136: ...on Uniformity Test When you run the temperature non uniformity test record the setpoint values for the wells listed on this data sheet At the end of the test compare the values that are displayed on the thermal cycler with the values that are recorded here Note You can photocopy this page Date Tested by Probe serial no Thermometer serial no Measurement Block TNU 1 at 95 C Block TNU 2 at 60 C 1 2 3...

Page 137: ... that has been moistened with bleach solution 20 bleach 80 water IMPORTANT Do not submerge the probe in bleach solution Do not disassemble the probe assembly CHEMICAL HAZARD Sodium hypochlorite bleach is a liquid disinfectant that can be corrosive to the skin and can cause skin depigmentation Read the MSDS and follow the handling instructions Wear appropriate protective eyewear clothing and gloves...

Page 138: ...A 4 Create a package that contains the Black case containing digital thermometer and probe assembly Address and contact information Billing address Shipping address Name and phone number of a contact preferably the person most familiar with the thermometer 5 Send the package freight prepaid to Alpha Technics at the address in step 1 ...

Page 139: ...Temperature Verification System For GeneAmp PCR Systems 2720 9600 9700 9800 and Veriti System User Guide B Instrument Warranty Information B This chapter covers Limited product warranty 132 Damages claims and returns 134 ...

Page 140: ... permitted to do so the warranty of the original manufacturer for such accessories With the exception of consumable and maintenance items replaceable products or components used on or in the instrument are themselves warranted to be free of defects in materials and workmanship for a period of ninety 90 days Applied Biosystems warrants that chemicals and other consumable products will be free of de...

Page 141: ... S SOLE AND EXCLUSIVE REMEDIES WITHOUT LIMITING THE GENERALITY OF THE FOREGOING IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE WHETHER IN CONTRACT TORT WARRANTY OR UNDER ANY STATUTE INCLUDING WITHOUT LIMITATION ANY TRADE PRACTICE UNFAIR COMPETITION OR OTHER STATUTE OF SIMILAR IMPORT OR ON ANY OTHER BASIS FOR DIRECT INDIRECT PUNITIVE INCIDENTAL MULTIPLE CONSEQUENTIAL OR SPECIAL DAMAGES SUSTAINED BY...

Page 142: ...nd request inspection by a local agent Secure a written report of the findings to support any claim Do not return damaged goods to Applied Biosystems without first securing an inspection report and contacting Applied Biosystems Technical Support for a Return Authorization RA number Claims After a damage inspection report is received by Applied Biosystems Applied Biosystems will process the claim u...

Page 143: ... TVK test is to detect any system failure that may result in a shift in the original manufacturer s calibration This kit should be used for temperature verification not system calibration For the GeneAmp 9700 the system has an aggregate accuracy of 0 25 C The TVK has accuracy and discrepancy in addition to the original manufacturing specification Temperature calibration of Applied Biosystems therm...

Page 144: ...136 Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720 9600 9700 9800 and Veriti System User Guide Appendix C ...

Page 145: ... Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720 9600 9700 9800 and Veriti System User Guide D Safety This appendix covers Instrumentation safety 138 Symbols on instruments 138 ...

Page 146: ...tes a standby switch by which the instrument is switched on to the Standby condition Hazardous voltage may be present if this switch is on standby Indicates the On Off position of a push push main power switch Indicates a terminal that may be connected to the signal ground reference of another instrument This is not a protected ground terminal Indicates a protective grounding terminal that must be...

Page 147: ...icates the presence of a hot surface or other high temperature hazard and to proceed with appropriate caution Indicates the presence of a laser inside the instrument and to proceed with appropriate caution Indicates the presence of moving parts and to proceed with appropriate caution Indicates the presence of a biological hazard and to proceed with appropriate caution Indicates the presence of a r...

Page 148: ...140 Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720 9600 9700 9800 and Veriti System User Guide Appendix D Safety ...

Page 149: ...20 18 19 system 9600 114 115 system 9700 32 33 system 9700 with 0 5 mL sample block module 88 89 system 9700 with Auto Lid sample block module 74 75 system 9700 with dual 384 well sample block module 60 61 system 9700 with dual 96 well sample block module 47 48 system 9800 103 104 system Veriti 127 128 digital thermometer 5 6 Calibration Verification Test 50 E equipment overview 2 H hazard icons S...

Page 150: ... 59 system 9700 with dual 96 well sample block module 42 46 system 9800 97 102 system Veriti 123 126 Temperature Verification System 2 4 tests Calibration Verification Test 9 12 23 26 37 41 64 68 78 82 92 96 119 122 Temperature Non Uniformity Test 13 17 27 31 42 46 55 59 69 73 83 87 97 102 123 126 text conventions viii training information on viii U user attention words described viii W WARNING de...

Page 151: ...sales office locations and technical support please call our local office or refer to our Web site at www appliedbiosystems com Applied Biosystems is committed to providing the world s leading technology and information for life scientists Headquarters 850 Lincoln Centre Drive Foster City CA 94404 USA Phone 1 650 638 5800 Toll Free In North America 1 800 345 5224 Fax 1 650 638 5884 08 2009 Part Nu...

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