Applied Biosystems 2720, User Manual

Page: 80 / 151

72 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 6 Temperature non-uniformity test
4. Press Enter . The GeneAmp
®
9700 automatically begins the second reading
(37 °C setpoint). The TNU Performance screen opens with the setpoint value
displayed.
Note:
The sample blocks must be within
±
1.0 °C of the setpoint.
5. Repeat steps 2 and 3 and for the second reading.
6. Press
Enter
. The TNU Performance screen opens with the following prompt:
7. Press and release the red button to automatically open the heated cover. Repeat
steps 4 through 6 of “Setting up the 20-µL probe assembly” on page 69 and
steps 1 through 6 of this procedure. Complete these steps for all 12 wells to be
tested:
8. The GeneAmp
®
9700 evaluates the uniformity of the sample block temperature
for the setpoint values that you entered and displays the results. A summary
screen opens at the end of the test.
If you entered values on the Temperature Non-Uniformity Test Data Sheet,
compare those values with the actual test results.
9. Press F1 (Accept). To interpret the results, see “Evaluating the results” on
page 73 .
Left Sample Block Right Sample Block
L1, L2, L3, L4, L5, and L6 R1, R2, R3, R4, R5, and R6
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
Setpoint is 37°C
Sample must be within 1.0°C of setpoint
F1 F3 F4 F5 F2
Cancel
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
F1 F3 F4 F5 F2
Cancel
Place probe in well xx
Press Run
Run
Well
F1 F3 F4 F5 F2
Accept
Cancel
94 ° C 37 ° C
More
xx.x L1 xx.x
xx.x L2 xx.x
xx.x L3 xx.x
xx.x L4 xx.x
Well 94 ° C 37 ° C
xx.x L5 xx.x
xx.x L6 xx.x
xx.x R1 xx.x
xx.x R2 xx.x