53
Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 5
Calibration verification test
5. Press
ENTER
. The GeneAmp
®
9700 automatically begins the second reading
(45 °C
setpoint). The Calibration Verification screen opens with the setpoint
value displayed.
Note:
The cover must be within
±
1 °C of 105 °C.
and
for the second reading.
7. The GeneAmp
®
9700 evaluates the calibration of the sample block temperature
for the setpoint values that you entered and displays the results. A summary
screen opens at the end of the test.
If you entered values on the Calibration Verification Test Data Sheet, compare
those values with the actual test results.
8. Press
F1
(Accept). To interpret the results, see
.
9. When you complete all measurements, be sure to:
• Press
Exit
.
• Remove the 0.02-mL probe assembly from the sample blocks.
• Power off the digital thermometer, then clean off the oil.
• Remove the 384-well temperature verification plates from the sample
blocks.
BURN HAZARD.
Make sure the sample blocks have cooled
at least to room temperature (less than 25 °C) before removing the probe. The
blocks and probes retain heat longer than expected.
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
Setpoint is 45°C
Cover must be within 1°C of 105°C
F1
F3
F4
F5
F2
Cancel
Calibration Verification
Actual temperature at
85 °C
Actual temperature at
45 °C
F1
F3
F4
F5
F2
Accept
Cancel
xx.x
xx.x
