Applied Biosystems 2720, User Manual

Page: 119 / 151

111 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 9 Temperature non-uniformity test
Temperature non-uniformity test
Use this test to verify the temperature uniformity of the GeneAmp
®
9600. The
temperature non-uniformity test consists of the following procedures, which you
must perform in sequence:
Setting up the 0.2-mL probe assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Running the test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Calculating test results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Ending the test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
PHYSICAL INJURY HAZARD. Hot Surface. Use care when
working around the heated cover and sample block to avoid being burned by hot
components.
Required
equipment
This test requires the 0.2-mL Temperature Verification Kit (PN 4317939), which
includes:
• Cotton swabs
• Light mineral oil
• 9600 temperature verification frame
• 0.2-mL probe assembly
• Model 4500 digital thermometer with a 9V battery installed
Setting up the
0.2-mL probe
assembly
1. If the heated cover is in the forward position, turn the knob completely
counterclockwise, then slide the heated cover back.
2. Using a cotton swab, coat the following wells with mineral oil:
A1, A4, A8, A12, C1, C4, C8, C12, E1, E4, E8, E12, H1, H4, H8, H12
3. Place the 9600 temperature verification frame on the sample block with the
channel facing the front of the instrument.
4. Place the 0.2-mL probe assembly into well A1.
5. Thread the probe wire through the channel in the 9600 temperature verification
frame to prevent damage to the probe and lead wires. Make sure the probe is
connected to the digital thermometer (see
Figure 18 on page 106 ).
6. Slide the heated cover forward, then turn the cover knob clockwise until the
white mark on the knob is aligned with the white mark on the cover.
IMPORTANT! Seat the probe properly and the close the heated cover carefully.
If the probe wire is crushed when you close the heated cover, the probe can be
damaged.
7. Power on the digital thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the digital thermometer, Model 4500.
Running the test
1. Power on the GeneAmp
®
9600.
For best results, allow the thermal cycler 15 to 30 minutes to warm up to
ambient temperature before performing the test.