80
Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 7
Calibration verification test
Running the test
Use the digital thermometer to take temperature readings of the sample well that is
connected to the 0.5-mL probe assembly. You must take readings at two different
setpoint temperatures.
Note:
If necessary, press
F5
(Cancel) to exit the test.
To run the calibration verification test:
2. Press
F1
(Run) to start the calibration verification test. The Calibration
Verification screen opens with the setpoint value displayed.
Note:
The cover must be within
±
1 °C of 105 °C. It may take several minutes
for the GeneAmp
®
9700 to ramp up.
3. The Calibration Verification screen counts down the time until the setpoint is
reached. When the “Stabilizing at setpoint”
value decreases to 0, read the digital
thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the digital thermometer, Model 4500.
4. Using the numeric keys, enter the value that is displayed on the digital
thermometer in the “Enter actual block temperature” field.
Note:
The digital thermometer displays a four-digit value; round this off to
three digits before typing it in the Calibration Verification screen.
Note:
Record this value on the Calibration Verification Test Data Sheet
(
) to keep a permanent record of the test.
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
Setpoint is 85°C
Cover must be within 10°C of 85°C
F1
F3
F4
F5
F2
Cancel
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
Stabilizing at setpoint... x:xx
F1
F3
F4
F5
F2
Cancel
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
F1
F3
F4
F5
F2
Cancel
Enter actual block temperature xx.x
