Applied Biosystems 2720, User Manual

Page: 107 / 151

99 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 8 Temperature non-uniformity test
Running the test
The temperature non-uniformity test uses the 0.1-mL probe assembly to test the
temperature uniformity of six different wells in each sample block.
Note:
If necessary, press F5 (Cancel) to exit the test.
To run the temperature non-uniformity test:
1. Complete the procedures in “Setting up the 0.1-mL probe assembly” on
page 97 .
2. Press F1 (Run) to start the temperature non-uniformity test. The TNU
Performance screen opens with the setpoint value displayed.
The TNU Performance screen counts down the time until the setpoint is stable.
Note:
The sample blocks must be within
±
1.0 °C of the setpoint. In addition,
the cover must be within
±
1 °C of 55 °C. It may take several minutes for the
GeneAmp
®
9800 to ramp up.
3. When the TNU Performance screen opens to display the setpoint temperature,
press F5 (Cancel).
4. When the “Stabilizing at setpoint” value decreases to 0, read the digital
thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the digital thermometer, Model 4500.
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
Setpoint is 55°C
Sample must be within 1.0°C of setpoint
F1 F3 F4 F5 F2
Cancel
Run
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
Stabilizing at setpoint... x:xx
F1 F3 F4 F5 F2
Cancel
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
Setpoint is 55°C
F1 F3 F4 F5 F2
Cancel
Sample must be 1.0°C of setpoint