25
Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 3
Temperature calibration verification test
2. When the “Stabilizing at setpoint”
value decreases to 0, read the digital
thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the digital thermometer, Model 4500.
3. Using the numeric keys, enter the value that is displayed on the digital
thermometer in the “Enter actual block temperature” field.
The digital thermometer displays a four-digit value. Round this off to three
digits before typing it in the Calibration Verification screen.
Record this value on the Calibration Verification Test Data Sheet (
) to
keep a permanent record of the test.
4. Press
Enter
.
The thermal cycler automatically begins the second temperature reading (45 °C
setpoint). The Calibration Verification screen displays the setpoint value.
Note:
The cover must be at 45 °C
±
30 °C. It may take several minutes for the
system to ramp up.
for the second temperature reading.
The thermal cycler evaluates the calibration of the sample block temperature for
the setpoint values that you entered, then displays the results. A summary
section opens when the test ends.
6. If you entered values on the Calibration Verification Test Data Sheet (
compare those values with the actual test results.
7. Press
F1
(Accept).
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
F1
F3
F4
F5
F2
Cancel
Enter actual block temperature xx.x
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
Setpoint is 45°C
Cover must be within 30°C of 45°C
F1
F3
F4
F5
F2
Cancel
Calibration Verification
Actual temperature at 85
°
C
Actual temperature at 45
°
C
F1
F3
F4
F5
F2
Accept
Cancel
xx.x
xx.x
