Applied Biosystems 2720, User Manual

Page: 47 / 151

39 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 4 Calibration verification test
Running the test
In this test, you take temperature readings of the sample well at two different set
points using the 0.2-mL probe assembly.
Note:
Press F5 (Cancel) if you want to exit the test.
To run the calibration verification test:
1. Press F1 (Run). The Calibration Verification screen opens with the setpoint
value displayed.
Note:
The cover must be at 105 °C
±
1 °C. It may take several minutes for the
system to ramp up.
The Calibration Verification screen counts down the time until the setpoint is
reached.
2. When the “Stabilizing at setpoint” value decreases to 0, read the digital
thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the digital thermometer, Model 4500.
3. Using the numeric keys, enter the value that is displayed on the digital
thermometer in the “Enter actual block temperature” field.
Note:
The digital thermometer displays a four-digit value; round this off to
three digits before typing it in the Calibration Verification screen.
Note:
Record this value on the Calibration Verification Test Data Sheet
( page 47 ) to keep a permanent record of the test.
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
Setpoint is 85°C
Cover must be within 1°C of 105°C
F1 F3 F4 F5 F2
Cancel
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
Stabilizing at setpoint x:xx
F1 F3 F4 F5 F2
Cancel
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
F1 F3 F4 F5 F2
Cancel
Enter actual block temperature xx.x