Applied Biosystems 2720, User Manual

Page: 100 / 151

92 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 8 Calibration verification test
Calibration verification test
Use the calibration verification test to verify the temperature calibration of your
Applied Biosystems GenAmp 9800 Fast Thermal Cycler with 96-Well Aluminum
Sample Block Module. The calibration verification test consists of the following
procedures, which you must perform in sequence:
Setting up the 0.1-mL probe assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Configuring the GeneAmp
®
9800 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Running the test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Evaluating the results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
Required
equipment
This test requires the following items in your Temperature Verification kit
(PN 4351630):
• Digital thermometer Model 4500 with a 9V battery installed
• Cotton swabs
• Light mineral oil
• 0.1-mL temperature verification probe assembly
Setting up the
0.1-mL probe
assembly
1. If the heated cover is in the forward position, lift the lever, then slide the heated
cover back.
2. With the instrument powered off and the sample block at room temperature,
place the 0.1-mL temperature verification probe assembly on the sample blocks.
Make sure the undercut of the 0.1-mL temperature verification probe assembly
is facing outwards.
3. Use a cotton swab to coat well A6 with mineral oil.
4. Place the 0.1-mL temperature verification probe assembly into well A6.
5. Thread the probe wire through the undercut of the 0.1-mL temperature
verification probe assembly to prevent damage to the probe and lead wires.
6. Make sure the probe is connected to the digital thermometer.
7. Slide the heated cover forward and pull the lever down.
IMPORTANT! Seat the probe properly, and close the heated cover carefully. If
the probe wire is crushed when you close the heated cover, the probe may be
damaged.
8. Power on the digital thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the digital thermometer, Model 4500.
9. Continue with “Configuring the GeneAmp
®
9800” on page 93 .