Applied Biosystems 2720, User Manual

Page: 105 / 151

97 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 8 Temperature non-uniformity test
Temperature non-uniformity test
Use this test to verify the temperature uniformity of the Applied Biosystems
GeneAmp
®
9800 Fast Thermal Cycler with 96-Well Aluminum Sample Block
Module. The temperature non-uniformity test consists of the following procedures,
which you must perform in sequence:
Setting up the 0.1-mL probe assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Configuring the GeneAmp
®
9800 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Running the test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Evaluating the results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
Required
equipment
This test requires the following items in your Temperature Verification Kit:
• Digital thermometer Model 4500 with a 9V battery installed
• Cotton swabs
• Light mineral oil
• 0.1-mL temperature verification probe assembly
Setting up the
0.1-mL probe
assembly
1. If the heated cover is in the forward position, lift the lever, then slide the heated
cover back.
2. With the instrument powered off and the sample block at room temperature,
place the 0.1-mL temperature verification probe assembly on the sample block.
3. Use a cotton swab to coat wells A1, A12, C4, C9, F4, F9, H1, and H12 with
mineral oil.
4. Place the 0.1-mL probe assembly into well A1.
As the test progresses, move the 0.1-mL probe assembly to each of the test wells
(A1, A12, C4, C9, F4, F9, H1, and H12).
5. Thread the probe wire through the undercut of the 0.1-mL probe assembly to
prevent damage to the probe and lead wires.
6. Make sure the probe is connected to the digital thermometer.
7. Slide the heated cover forward and pull the lever down.
IMPORTANT! Seat the probe properly and close the heated cover carefully. If
the probe wire is crushed when you close the heated cover, the probe may be
damaged.
8. Power on the digital thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the digital thermometer, Model 4500.
9. Continue with “Configuring the GeneAmp
®
9800” on page 98 .