Applied Biosystems 2720, User Manual

Page: 52 / 151

44 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 4 Temperature non-uniformity test
Running the test
The temperature non-uniformity test uses the 0.2-mL probe assembly to test the
temperature uniformity of 6 different wells in each sample block.
Note:
Press F5 (Cancel) if you want to exit the test.
To run the temperature non-uniformity test:
1. Press F1 (Run). The temperature non-uniformity test starts, and the TNU
Performance screen opens with the setpoint value displayed.
Note:
The sample block must be at 95 °C
±
1.0 °C. In addition, the cover must
be at 105 °C
±
1.0 °C. It may take several minutes for the GeneAmp
®
9700 to
stabilize at the setpoint temperature.
The TNU Performance screen counts down the time until the setpoint is stable.
2. When the “Stabilizing at setpoint” value decreases to 0, read the digital
thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the digital thermometer, Model 4500.
3. Using the numeric keys, enter the value that is displayed on the digital
thermometer in the “Enter actual block temperature” field.
Note:
The digital thermometer displays a four-digit value; round this off to
three digits before typing it in the TNU Performance screen.
Note:
Record this value on the Temperature Non-Uniformity Test Data Sheet
( page 48 ) to keep a permanent record of the test.
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
Setpoint is 95°C
Sample must be within 1.0°C of setpoint
F1 F3 F4 F5 F2
Cancel
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
Stabilizing at setpoint x:xx
F1 F3 F4 F5 F2
Cancel
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
F1 F3 F4 F5 F2
Cancel
Enter actual block temperature 00.0