52
Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 5
Calibration verification test
To run the calibration verification test:
2. Press
F1
(Run) to start the calibration verification test. The Calibration
Verification screen opens with the setpoint value displayed.
Note:
The cover must be within
±
1 °C of 105 °C. It may take several minutes
for the GeneAmp
®
9700 to ramp up.
3. The Calibration Verification screen counts down the time until the setpoint is
reached.
When the “Stabilizing at setpoint”
value decreases to 0, read the digital
thermometer. Refer to the instructions in your Temperature Verification Kit for
details on operating the digital thermometer, Model 4500.
4. Using the numeric keys, enter the value that is displayed on the digital
thermometer in the “Enter actual block temperature” field.
Note:
Round off the four-digit value on the digital thermometer to three digits
before you enter it into the Calibration Verification screen.
Note:
Record this value on the Calibration Verification Test Data Sheet
(
) to keep a permanent record of the test.
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
Setpoint is 85°C
Cover must be within 1°C of 105°C
F1
F3
F4
F5
F2
Cancel
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
Stabilizing at setpoint... x:xx
F1
F3
F4
F5
F2
Cancel
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
F1
F3
F4
F5
F2
Cancel
Enter actual block temperature xx.x
