Applied Biosystems 2720, User Manual

Page: 77 / 151

69 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 6 Temperature non-uniformity test
Temperature non-uniformity test
Use this test to verify the temperature uniformity of the GeneAmp
®
PCR System
9700, Auto-Lid Sample Block Module. The temperature non-uniformity test consists
of the following procedures, which you must perform in sequence:
Setting up the 20-µL probe assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Configuring the GeneAmp
®
9700 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Running the test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Evaluating the results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
PHYSICAL INJURY HAZARD. Hot Surface. Use care when
working around the heated cover and sample block to avoid being burned by hot
components.
Required
equipment
This test requires the Dual 384-Well Sample Block Module Temperature Verification
Kit (PN 4308354), which includes:
• Light mineral oil
• Two 384-well temperature verification plates
• 20-µL probe assembly
• Digital thermometer with a 9V battery installed
Note:
This kit is also used for the Auto-Lid sample block module.
Setting up the
20-µL probe
assembly
1. Make sure the heated cover is in the rear position. If not, press and release the
red button to automatically move the heated cover to the rear position.
2. Place both 384-well temperature verification plates on the sample blocks.
IMPORTANT!
The temperature verification plates must be installed in both
sample blocks throughout all temperature verification testing.
The plate labeled L1 to L6 is for the left sample block (Fig 14). The plate
labeled with R1 to R6 is for the right sample block (Fig 15). Position the plates
so the angled corners face away from you.
Note:
The plates help you locate the probes within the sample blocks.
Figure 16 Left temperature
verification plate
Figure 17 Right temperature
verification plate