Applied Biosystems 2720, User Manual

Page: 91 / 151

83 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 7 Temperature non-uniformity test
Temperature non-uniformity test
Use this test to verify the temperature uniformity of the
GeneAmp
®
PCR System
9700
,
0.5-mL Sample Block Module. The temperature non-uniformity test consists
of the following procedures, which you must perform in sequence:
Setting up the 0.5-mL probe assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Running the test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Evaluating the results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Required
equipment
This test requires the 0.5-mL Sample Block Module Temperature Verification Kit
(PN 4309924), which includes:
• Cotton swabs
• Light mineral oil
• GeneAmp
®
0.5-mL temperature verification frame
• 0.5-mL probe assembly
• Digital thermometer Model 4500 with a 9V battery installed
Setting up the
0.5-mL probe
assembly
1. If the heated cover is in the forward position, lift the lever, then slide the heated
cover back.
2. Place the GeneAmp
®
0.5-mL thermal insulation frame on the sample block.
3. Use a cotton swab to coat the following wells with mineral oil:
A1, A6, A10, B3, C5, C10, D1, D7, E8, F1, F5, F10
4. Place the 0.5-mL probe assembly into well A1.
Note:
As the test progresses, you move the 0.5-mL probe assembly to each of
the test wells.
5. Thread the probe wire through the channel in the 0.5-mL temperature
verification frame to prevent damage to the probe and lead wires.
6. Make sure the probe is connected to the digital thermometer.
7. Slide the heated cover forward and pull the lever down.
IMPORTANT!
Seat the probe properly and close the heated cover carefully. If
the probe wire is crushed when you close the heated cover, the probe may be
damaged.
8. Power on the digital thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the digital thermometer, Model 4500.
9. Continue with “Configuring the GeneAmp
®
9700” below.
Configuring the
GeneAmp
®
9700
1. Complete the procedures in “Setting up the 0.5-mL probe assembly” above.
2. Power on the thermal cycler. The Main menu opens.
For best results, allow the thermal cycler 15 to 30 minutes to warm up to
ambient temperature before performing the test.