Thermo Scientific Heracell 150i, Руководство по эксплуатации

Страница: 38 / 156

Operating Instructions | HERAcell® 150i / 240i
4 Description of the device 4 Description of the device
38
50115191 A / 08.2009
4.5 Safety devices
The device is equipped with the following safety devices:
• A door switch interrupts the CO
2
/O
2
/N
2
supply and the work space
heating when the glass door is opened.
• The optional gas detector switches the gas supply over to a full gas
cylinder.
• An independent thermal protection protects the samples from harmful
overheating in case of failures.
• A pressure compensation opening ensures pressure compensation in
the device work space.
• The alarm relay circuit uses audible and visual alarms to indicate errors
during operation.
4.6 Work space atmosphere
In the work space of the incubator, the particular physiological ambient
conditions for the preparation and cultivation of cell and tissue cultures are
simulated. The work space atmosphere is determined by the following
factors:
• Temperature
• Relative humidity
• CO
2
concentration
• O
2
concentration (optional)
Temperature:
Temperature conditions
To ensure undisturbed operation, the temperature in the operating room
must be at least 18 °C and the incubation temperature must exceed this
temperature by at least 3 °C.
The heating system controls the incubation temperature from this
temperature threshold up to 55 °C. The principle of air jacket heating and
the additional, separate heating of the outer door and glass door/gas-tight
screen minimize the risk of condensate formation at the side walls, at the
ceiling of the work space, and at the glass door/gas-tight screen.
Relative humidity:
Water quality
The heating of the work space promotes the condensation of the water,
thereby ensuring a constant humidity within the work space. For the
running operation, always keep a sufficient quantity of processed water of
the following quality available:
• HERAcell
®
240i: quantity 4.5 l,
• HERAcell
®
150i: quantity 3.0 l,
Water quality recommendation:
For trouble-free operation of the equipment, fill the water reservoir with
sterilized destilled water or equivalent. The acceptable conductivity should
be within the range of 1 to 20
μ S (resistivity within the range of 50 k Ω to
1 M Ω ).