Thermo Scientific Heracell 150i, Руководство по эксплуатации

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Operating Instructions | HERAcell® 150i / 240i
4 Description of the device 4 Description of the device
39
50115191 A / 08.2009
Termination of warranty!
Using chlorinated tap water or additives that contain chlorine
will void the manufacture warranty. Similarly, the use of
ultrapure water whose conductivity is out of the range of 1 to
20
μ S (and whose resistivity is out of the range of 50 k Ω to
1 M Ω ) will void the manufacture warranty.
If you should have any questions, please contact Technical
Support of Thermo Fisher Scientific.
Under normal operating conditions and at the usual incubation
temperature of 37 °C, a constant relative humidity of approx 93% is
achieved in the work space.
If dew formation occurs on the culture containers due to
elevated relative humidity, the humidity in the work space can be adapted
to a lower value by enabling the Low Humidity feature. The relative
humidity in the work space is lowered from approx. 93% to approx. 90%.
The modification requires an extended adaption phase. To ensure that it
effectively prevents
dew formation on culture containers, it must be used as a permanent
setting.
CO
2
supply:
CO
2
-Work space atmosphere control
To ensure the growth conditions for the cell and tissue cultures, the work
space is supplied with CO
2
.
The pH of the bicarbonate-buffered culture media largely depends on the
CO
2
content of the work space atmosphere.
The CO
2
content of the work space atmosphere can be controlled within a
range of 0 to 20%.
The supplied CO
2
must have one of the following quality characteristics:
• Purity 99.5% min.,
• medical gas quality.
O
2
supply:
O
2
-Work space atmosphere control
If the CO
2
incubator is to be operated with more than 21% oxygen, the
work space is supplied with oxygen. The O
2
content of the work space
atmosphere can be controlled within a range of 0 to 21%.
N
2
supply:
N
2
-Work space atmosphere control
If the oxygen content during operation is to be lowered to less than 21%
(air oxygen content), the work space is supplied with nitrogen. The O
2
content of the work space atmosphere can be controlled depending on the
configuration of the sensor within a range of 1% to 21% and 5% to 90%.