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LISST-100X User’s Guide

specifying raw data
file and all
information needed
for data processing.



Choose the serial number of the instrument that

collected the data to be processed.

Instrument serial
number selected.

Only the serial numbers of the instruments loaded into the
InstrumentData.Txt file and have the calibration values
loaded into the LISST.INI file in the Windows directory
can be used.



Select the background scatter data file from the file

selection window.

Background
scatterfile selected.

Double click the file or type the file name and press
Open.

Содержание LISST-100X

Страница 1: ...User s Manual Version 5 0 2700 Richards Road Suite 107 Bellevue WA 98005 4200 Tel 425 641 0944 Fax 425 643 0595 LISST 100X Particle Size Analyzer...

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Страница 3: ...This document is copyrighted by SEQUOIA SCIENTFIC INC...

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Страница 6: ...ce For technical assistance please contact your local Distributor or Sequoia Please be sure to include the instrument serial number with any correspondence IMPORTANT Please read APPENDIX H TECHNICAL A...

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Страница 8: ...Waste Electrical and Electronic Equipment Smaltimento di apparecchiature elettriche ed elettroniche da rottamare...

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Страница 11: ...ed Real time data processing 64 Step by Step Procedure Configuring data collection 69 INSTRUMENT COMMUNICATION 75 LISST 100X COMMAND SUMMARY 79 LISST 100X COMMAND DETAILS 81 PERFORMANCE OPTIMIZATION 9...

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Страница 13: ...of laser light is observed at multiple small forward angles At these small angles light scattering is determined almost entirely by light diffracted by the particle The light transmitted through the p...

Страница 14: ...o size distribution involves a mathematical inversion In this step a size distribution is found that would produce multi angle scattering that fits the observation There exists a vast literature on th...

Страница 15: ...we have provided software that obtains the inverted volume distribution NV 1 Agrawal Y C I N McCave and J B Riley 1991 Laser diffraction size analysis in Principles methods and applications of particl...

Страница 16: ...ded in the Windows software For MATLAB users inversion scripts are available for download from Sequoia s website www SequoiaSci com The principal measurement angular scattering distribution is obtaine...

Страница 17: ...large such as typically happens during profiling applications External I O Ports The LISST 100X is also equipped with an external ANALOG IN port for recording the output of one other analog instrument...

Страница 18: ...to 24 VDC external power Cables up to 50 meters can be provided to supply external power and communication with the instrument This can allow real time observation of the size distributions When not u...

Страница 19: ...LISST 100X User s Guide 7...

Страница 20: ...8 LISST 100X User s Guide...

Страница 21: ...nstrument please handle it gently as you would handle a very expensive camera Critical alignments may be disturbed if the instrument is subjected to shock or rough handling Evidence of shock rough han...

Страница 22: ...ower cable and Allen wrench set Step 1 Remove Instrument from shipping case Start by removing the white plastic instrument stands and set them on a flat working surface Remove the LISST 100X from the...

Страница 23: ...k Use 5 pin connector Used for serial communication and external power See Appendix C Connector Pinouts for LISST 100X 6 pin connector Used for analog in and digital in out signals See Appendix C Conn...

Страница 24: ...ons Cable Remove the Communications cable from the shipping crate It is the 2 meter cable with the 9 pin DB 9 connector on one end and the 5 pin underwater connector on the other Remove the underwater...

Страница 25: ...rogram Data Sequoia LISST100 This is the default folder for saving your data but you can change it in the software There are 2 very important files in this folder InstrumentData txt and LISST ini Thes...

Страница 26: ...ment status will be displayed If the cable connections are not correct or if the battery is not installed a warning message will appear after the time expires The serial port settings can be checked b...

Страница 27: ...ill print ZZZ to the screen every 30 seconds To wake the instrument from the Terminal window press the Stop button immediately after the ZZZ is displayed The instrument will only respond to the Stop b...

Страница 28: ...strument The image below shows an example of this display It is opened by either selecting Collect Background Scatter Data from the LISST menu or by pressing the button on the toolbar After selecting...

Страница 29: ...Laser power value can also be an indication of optical misalignment Step 11 Configuring Instrument for Deployment The Operating Modes window is used to set all of the deployment parameters To open the...

Страница 30: ...ution displays This mode sends the raw data to the LISST SOP software for conversion to Size Distribution and displaying on the screen No data is saved on board the instrument when this mode is used B...

Страница 31: ...ed Choose the Start Condition tab at the top of the window There are five options Depth Time External Mechanical Switch External Digital Input and Time Delay Select the mode by clicking on the button...

Страница 32: ...en an LOP extension which will identify it as a LISST Operating Procedure File Comments can be entered before saving in the box below the summary Previously saved LOP files can be opened using the Bro...

Страница 33: ...inning the data collection Step 17 Background file warning If the background file you selected is more than 6 hours old a File Warning dialogue box will appear alerting you that final data quality cou...

Страница 34: ...being flicked to the 1 position Only then will actual sampling begin If you select the No button the warning below appears informing you how you can start the instrument When the instrument has been s...

Страница 35: ...h Start and Depth Stop For all other Start Conditions the program will terminate and return to the L100X prompt To stop a running program use the Stop button on the Terminal window or the button on th...

Страница 36: ...a Transfer Status window will appear The data is offloaded at 115K baud If you click the cancel button in the upper right corner of the transfer window the current file transfer and any remaining file...

Страница 37: ...select the background scatter file to use when processing the data The LISST SOP will do a check of the laser power value in the selected background scatter file If the laser power is 0 zero a warning...

Страница 38: ...created depends on the selections in the Settings selection in the File menu The software will automatically determine the Instrument Type B or C using the Serial Number and the InstrumentData Txt fi...

Страница 39: ...II files and as binary files with a PSD extension The PSD files can be opened and viewed on the screen To open a processed Particle Size Distribution file choose Open Particle Distribution File or pre...

Страница 40: ...Guide To view the value of the auxiliary parameters such as time and depth select the View Auxiliary Parameter Frame from the DataFrames menu A display similar to the one below will appear showing th...

Страница 41: ...he display will be reduced The Start and Stop conditions will not be used and therefore their settings are not relevant NOTE For extended Real Time sessions it is recommended to use external power The...

Страница 42: ...s not being used Pressing the next button will command the LISST to make a measurement and transmit the data to the SOP for display The Spherical Random Shape radio buttons can be selected in order to...

Страница 43: ...ment back to sleep After finishing with the instrument it should be put back into its low power sleep mode In this mode with the communication cable disconnected the drain on the battery is only about...

Страница 44: ...32 LISST 100X User s Guide of these steps are covered in detailed Step by Step instructions in the following sections...

Страница 45: ...g deployment follow these processing steps that includes optional displays and procedures Page 40 Batch Processing Multiple raw data files Data that has been downloaded from the datalogger is in a raw...

Страница 46: ...0 Advanced Real time data processing The advanced real time data processing allows for using all data collection modes and start stop options e g burst mode delayed start pressure start with the real...

Страница 47: ...ions program started L100X prompt showing 3 Open the Collect Background Scatter Data from the LISST menu or by pressing the button on the toolbar After selecting the factory background scatter file to...

Страница 48: ...some of the inner 6 rings are high 6 Note down the 33rd and the 36th value they should be around 1000 1500 for most LISST 100X s Element 33 factory Element 36 factory Elements 33 and 36 from the fact...

Страница 49: ...the file name on the list Multiple files can be selected by hold down the CTRL key while clicking on files Use the SHIFT key to select a range of files Files selected and path set Press the OK button...

Страница 50: ...se errors during offloading If an offload error occurs you can change the offload baud rate as follows 6 Go to Settings in the File Menu and click the Serial Port tab In the File Offload Baud Rate dro...

Страница 51: ...ISST menu A list of files on the datalogger will appear 3 Select the files to be deleted then click the OK button Use the CTRL key to select multiple files or the SHIFT key to select a range of files...

Страница 52: ...and calibrated data This is useful for loading into spreadsheets such as Excel etc The LOG file is a space delimited ASCII file containing the raw data from the LISST 100X data logger This is useful f...

Страница 53: ...e the Spherical particle model The reason for this is that no other laser manufacturer provides a randomly shaped particle model for inversion so there will be nothing to compare to Generally it is re...

Страница 54: ...d Instrument serial number selected Only the serial numbers of the instruments loaded into the InstrumentData Txt file and have the calibration values loaded into the LISST INI file in the Windows dir...

Страница 55: ...same base name but with different extensions Note file types other than the default PSD file must have been selected before starting the Open Raw Data file command See step 2 Output data file name se...

Страница 56: ...yed at a time Use the Next and Previous buttons to move through the complete datafile The triangles on the lower edge of the upper display will indicate where in the complete datafile the currently di...

Страница 57: ...creen will show the current sample or frame Pressing the Select First button will set the current cursor position as the starting point for processing Similarly selecting Select Last will set the end...

Страница 58: ...SCII file containing all the processed data The LOG file is a space delimited ASCII file containing the raw data from the LISST 100X data logger Select file types to be created 3 You must also select...

Страница 59: ...manufacturer provides a randomly shaped particle model for inversion so there will be nothing to compare to Generally it is recommended that you keep both boxes checked this is also the default optio...

Страница 60: ...d have the calibration values loaded into the LISST INI file in the Windows directory can be used 6 Select the background scatter file zscat file to be used for processing your data Click the select b...

Страница 61: ...LISST 100X User s Guide 49...

Страница 62: ...another directory The Raw Files List box now shows all the raw data files DAT or LOG extension that are in your Raw Files Directory while the Output Files Box shows ASC PSD and LOG files that may alr...

Страница 63: ...6 processed files for each raw data file An ASC PSD and LOG file for both spherical and random shape inversions Note that the files processed using the randomly shaped inversion method have an _rs su...

Страница 64: ...the command line The paths and file names are stored in the LISST INI file which resides in the C Program Data Sequoia LISST100 directory You can examine the LISST INI file before and after batch pro...

Страница 65: ...LISST 100X User s Guide 53 Notes...

Страница 66: ...tafile selected 4 After selecting the processed file a window similar to the one shown below will appear The left hand display is the volume distribution in units of micro liters liter in each size cl...

Страница 67: ...this window is shown below 7 The scale of the Volume Distribution plot can be changed by using the Scale button After selecting the button a display similar to the one shown below will prompt you for...

Страница 68: ...urrent display can be sent to the printer by choosing Print from the File menu Print Preview and Print Setup are also available Display printed 9 When finished viewing the processed data close the win...

Страница 69: ...ument becomes older and to some extent with temperature Values around 0 5 2 mW are the most common values Laser Reference should never be 0 or very close to 0 e g 0 02 mW If this is the case the laser...

Страница 70: ...ues generally are in the 0 98 0 995 range your measurements are taken in very clear water This means that the signal to noise ratio will be low and the data may have a lot of noise in them but can mos...

Страница 71: ...LISST 100X User s Guide 59 Notes...

Страница 72: ...binary PSD file must be selected before any other file type will be stored This is used to display the processed data to the screen The ASC type is a spaced delimited file containing all the processed...

Страница 73: ...ons The slide button next to the Timer button controls the sample speed If it is set too fast it will automatically reset to the maximum allowed rate Timer button pressed and samples updating automati...

Страница 74: ...by selecting the View Rings command from the File menu An example of this display is shown below Value of rings for current frame displayed 11 A single sample may be saved to a separate file by pressi...

Страница 75: ...the window is closed However if the window is left open the power will remain on potentially draining substantial life from the battery To turn off the power without exiting the Real Time session pre...

Страница 76: ...g Open Real Time Session Select Output from the Options menu The window below will appear Select output settings A binary PSD file must be selected before any other file type will be stored This is us...

Страница 77: ...file Background and Output file selected 5 Open up the operating mode windows and select the desired operating mode In this case burst mode is being selected Instrument configured with the desired ope...

Страница 78: ...de can always be stopped by selecting the Stop button in the SOP window Stop condition selected 8 Now click Apply and then OK You will be asked if you wish to start the instrument It is very important...

Страница 79: ...ing the realtime data and select an output file name Then click OK and the realtime window will open up Check the Listen Mode check box Prepare real time window for listen mode 11 Next open the termin...

Страница 80: ...s being saved as default but it is very strongly recommended that the user also always selects a LOG file output option in File Settings Output tab before starting the real time processing Please note...

Страница 81: ...LISST menu or by pressing the button on the toolbar When first opening the Operating Modes window the LISST SOP program will send a Ctrl C to the LISST 100X If a program is running it will be stopped...

Страница 82: ...set the type of sampling RealTime Burst or Continuous You can also select the samples per average and sample rates on this screen The RealTime mode is used for real time size distribution displays Thi...

Страница 83: ...to configure when the instrument will begin sampling There are five options Depth Time External Mechanical Switch External Digital Input and Time Delay Select the mode by clicking on the button next t...

Страница 84: ...the Base Program on the instrument will return to waiting for the Start condition For example if the start and stop conditions were set to External Mechanical Switch then multiple sampling sessions ca...

Страница 85: ...6 hours old a warning is issued and the user is being given a choice between selecting a different file collecting a new background or accepting the risk and continue For best results the zscat file s...

Страница 86: ...al window The Run button is equivalent to typing RUN and pressing Enter from any terminal program 11 The Base Program will display text to the screen as it is running to confirm that it is running and...

Страница 87: ...screen and then return to sleep The instrument firmware is retained in on board flash memory and will start executing when power is applied After a power up pressing the ENTER key will cause an echo o...

Страница 88: ...is switch once a second If the switch is in the on or 1 positions the program will go directly to the data collection routine It will continue checking the switch status until the switch is moved to t...

Страница 89: ...s the instrument is moving up and down If the Start Condition is Depth Start the Base program will return to checking for the Start Conditions For all other Start Conditions the when the current depth...

Страница 90: ...aved has been reached the program will stop and return to the L100X prompt Maximum Memory or Low Battery The Maximum Memory or Low Battery Stop condition will continue to sample until the memory capac...

Страница 91: ...o x BI x Set Burst Interval SB x Set Samples per Burst SC mm dd yy hh mm ss Set Clock with time and date where mm month dd day yy year hh hour 24 format mm minute ss seconds Example ST 01 05 2005 21 0...

Страница 92: ...80 LISST 100X User s Guide consumption...

Страница 93: ...is 778B 155F Directory of C L040305_ BIN 4 136 03 05 04 6 30p L183705 BIN 1 672 03 05 04 6 37p 2 file s 5 808 bytes 0 dir s 15 933 440 bytes free L100x DS Display current status information Syntax DS...

Страница 94: ...f text Setup Commands OM Set Operating Mode Syntax OM x or om x Description Sets the Operating Mode to one of the following types 1 Real Time Mode 2 Burst Mode 3 Fixed Rate Mode OM command only withou...

Страница 95: ...hen the GO command is issued The start condition data is used with the Start Condition setting as follows If the Start Condition is Depth Start option 1 the input will be start depth in meters If the...

Страница 96: ...he input for TD will be the stop date and time If the Stop Condition is Fixed Number Stop option 5 the input will be the number of samples to collect before stopping The TD setting is ignored for Mech...

Страница 97: ...nute ss seconds If no values follow the SC or sc command prompts for entering the time and date will be displayed Example input SC 01 05 2001 21 05 03 output Command Data in SC is 01 05 01 21 05 03 Cu...

Страница 98: ...nd to verify the current settings Then confirm 1 yes 0 no that these settings should be burned into the EEPROM to be used with the AutoStart setting Example input SD output LISST 100X Current Status a...

Страница 99: ...ll be displayed as part of the DS status if the store mode is disabled WARNING Data storage disabled Data will not be saved ZD Reset Depth Sensor Offset Syntax ZD or zd Description The ZD or zd comman...

Страница 100: ...tax GX or gx Description Acquires single averaged sample and displays the result to the screen Example Input GX Output 32 ring values 8 Aux parameters Notes The GX command does not store the sample to...

Страница 101: ...hen acquiring a background Example Input ZS Output 32 ring values 8 Aux parameters repeat 20 times Notes The ZS command does not store the sample to a datafile ZZ Go into deep sleep mode minimum power...

Страница 102: ...les It is very important that clean and bubble free water is used The water can be fresh or salt water For most applications it has been found that steam distilled water is sufficient Steam distilled...

Страница 103: ...is very important that a good background file be obtained with very clean water As particle concentrations increase the relative signal to background noise ratio also increases thus reducing the impo...

Страница 104: ...ner rings are shown on the left side of the background display The most important rings for misalignment indication are rings 1 4 in the background scatter file As the focused laser starts to move awa...

Страница 105: ...aller diameter section is the suggest mounting area The U shaped mounting block provided with the instrument fit this diameter and can be used for mounting the instrument The clamps supplied with the...

Страница 106: ...is a sensitive instrument When not in use the instrument should be stored in its shipping case For longer storage year or so the communication cable should be disconnected and the battery pack should...

Страница 107: ...pressure and temperature sensors can be re calibrated if desired The performance of the instrument can be checked with the use of a sample of particles of a known size distribution Such samples ship a...

Страница 108: ...t remove the end cap by gently threading the long bolts in evenly They will bottom out against the edge of the pressure housing and slowly force out the connector endcap When the O ring becomes visibl...

Страница 109: ...t the position of the connectors to minimize the diameter of the assembly If the connectors are not arranged properly it will be difficult to insert the battery into the pressure case Battery wrapped...

Страница 110: ...98 LISST 100X User s Guide...

Страница 111: ...micron Resolution 32 size classes log spaced Data storage memory 1GB 12 million measurements standard Maximum sample speed 1 size distributions per second standard Temperature sensor range 10 to 45o C...

Страница 112: ...e in a text editor Auxiliary Parameter Display Label Multiplier Offset Units Parameter 1 Laser Power mW Parameter 2 Battery 0 01 0 volts Parameter 3 External Instrument volts Parameter 4 Laser Referen...

Страница 113: ...2 5 27 6 27 2 86 3 38 3 11 5 72 6 75 6 21 7 26 3 38 3 98 3 67 6 75 7 97 7 33 8 25 3 98 4 70 4 33 7 97 9 40 8 65 9 24 4 70 5 55 5 11 9 40 11 1 10 2 10 23 5 55 6 55 6 03 11 1 13 1 12 1 11 22 6 55 7 72 7...

Страница 114: ...5 14 4 73 7 26 2 70 3 19 2 93 5 14 6 06 5 58 8 25 3 19 3 76 3 46 6 06 7 15 6 59 9 24 3 76 4 44 4 09 7 15 8 44 7 77 10 23 4 44 5 24 4 82 8 44 9 96 9 17 11 22 5 24 6 18 5 69 9 96 11 8 10 8 12 21 6 18 7...

Страница 115: ...rst size range for type C randomly shaped particles bins 1 x rho 0 31 lower limit bins 2 x rho 1 32 upper limit bins 3 sqrt bins 1 bins 2 mid point B_all logspace 0 log10 200 33 0 1 create 33 logspace...

Страница 116: ...24 0 482 0 222 0 262 0 241 11 22 0 524 0 618 0 569 0 262 0 309 0 284 12 21 0 618 0 729 0 671 0 309 0 365 0 336 13 20 0 729 0 861 0 792 0 365 0 430 0 396 14 19 0 861 1 02 0 935 0 430 0 508 0 467 15 18...

Страница 117: ...0 465 0 428 0 197 0 232 0 214 12 21 0 465 0 548 0 505 0 232 0 274 0 252 13 20 0 548 0 647 0 596 0 274 0 324 0 298 14 19 0 647 0 764 0 703 0 324 0 382 0 351 15 18 0 764 0 901 0 829 0 382 0 451 0 415 16...

Страница 118: ...Elements Parameter 1 32 Light intensity on detectors 1 through 32 33 Laser transmission Sensor 34 Battery voltage in raw counts 35 External Auxiliary input 1 0 to 5V 0 to 4096 36 Laser Reference senso...

Страница 119: ...calibrated units mW 36 Laser Reference sensor in calibrated units mW 37 Pressure in calibrated units m 38 Temperature in calibrated units C 39 Day 100 Hour at which data taken 40 Minutes 100 Seconds a...

Страница 120: ...nnector Pinouts for LISST 100X The LISST 100X has 3 separate underwater connectors A 5 pin a 6 pin and a 3 pin connector The photograph shows the placement of each connector The following text describ...

Страница 121: ...an Diego CA USA Connector Part Number Bulkhead MCBH WB 5 MP Stainless Steel Mating Cable Part Number MCIL 5 FS Connector Pin Use 1 Power Serial Ground 2 External Power In 9V nom 6 15V 8 3 Power Serial...

Страница 122: ...rt Number MCIL 6 FS Connector Pin Use 1 Digital Ground 2 Digital In 1 3 Digital In 2 4 No Connection 5 Analog In 0 to 2 50V max 6 Analog Ground BioBlock Connector 3 pin connector Connector Manufacture...

Страница 123: ...t Clock Sets the internal instrument clock to match the currently connected computer clock Operating Modes Opens the Instrument Sampling Configuration window where operating mode and sampling schemes...

Страница 124: ...the Print button will be activated Pressing the button will send the current display to the printer Put LISST to sleep Attempts to wake up instrument from low power sleep mode This may take up to 40 s...

Страница 125: ...ip and replace files Unzip the file to a temporary folder on your hard drive and then REPLACE the following 4 four files with the files you already have on your PC Factory_zsc_2002 asc replace with th...

Страница 126: ...amber first clean the windows of the LISST 100X NOTE On older versions of the LISST 100X it may also be necessary to remove the endcap ring and standoffs on the optics endcap Remove the three screws o...

Страница 127: ...l of the chamber It may be necessary to press on the top bottom and sides of the Slider to get it to slide out without binding Step 5 Once the slider is positioned such that the O ring is engaging the...

Страница 128: ...BioBlock is self contained with its own battery pack It uses a 5V logic signal from the dedicated BioBlock connector on the LISST 100X connector endcap This appendix is separated into three parts The...

Страница 129: ...st for correctly positioning the unit during installation The BioBlock should be removed from the LISST 100X in the Closed position to facilitate re installing it at a later date The BioBlock may be c...

Страница 130: ...to hold the BioBlock in place Step 4 Slowly slide the BioBlock shutter assembly straight down between the Crossbar Standoffs and the windows the LISST 100X sample volume taking care not to drag the c...

Страница 131: ...to the LISST 100X and configure the instrument using the instructions on the following pages Then verify normal operation by cycling the system using the LISST EXE software The BioBlock shutter shoul...

Страница 132: ...xample issuing a BB 10 command will enable the connection and send the command to close the BioBlock A BB 11 command will also enable the connection and send an open command During normal sampling the...

Страница 133: ...LISST 100X User s Guide 121 command the BioBlock to remain open By monitoring the battery the BioBlock can be left in the open position before the battery voltage is too low...

Страница 134: ...a battery mount attached to the connector endcap Remove the batteries and replace with fresh alkaline batteries If desired wrap each battery with electrical tape to keep it in place during installatio...

Страница 135: ...annot use the psd or asc files produced by the LISST SOP for troubleshooting do not include these in your email We can only help you if you supply the information listed in 1 3 above Q When I try to q...

Страница 136: ...inside the cable The voltage should read 12 V If it does not the cable may be defect Short the female pins 4 and 5 on the communication cable using one or both paperclips Type some characters into MO...

Страница 137: ...re save the edited LISST INI file in C ProgramData Sequoia LISST100 Q When I try to offload the data I get an error and the offload stops I can talk to the instrument and program it but it will not of...

Страница 138: ...s The standard underwater connectors for communications and power are molded to the cable Full Path Mixing Chamber Allows the submersible LISST 100 100X to also be used as a laboratory particle size a...

Страница 139: ...measured without the need for permanent optical pathreductions PRMs are available to reduce the optical path by 50 80 and 90 Large Volume Test Chamber Horizontal acrylic chamber with O ring and drain...

Страница 140: ...128 LISST 100X User s Guide Replacement Zscat Chamber Used for doing background scatter measurement zscat before deployment One chamber ships as standard with the LISST 100X...

Страница 141: ...liable for normal wear and tear such as replacement of consumables nor for defects or failure caused by maintenance misuse negligence or failure resulting from non compliance with SEQUOIA s specifica...

Страница 142: ...o provide such indemnification 9 Statute of Limitations Customer will not commence any action based on breach of warranty with respect to any Product more than 18 months after SEQUOIA s delivery of su...

Страница 143: ...Digital Inputs 5 E Error Messages could not find Instrumentxxxx 125 no valid query response 123 External I O Ports analog 5 digital 5 76 77 External Power Supply 6 99 F File type asc 27 40 107 dat 10...

Страница 144: ...ndard Particles 95 Start Conditions 71 depth start 76 external digital input 76 external mechanical switch 76 time delay 76 time start 76 Stop Conditions 72 depth stop 77 external digital input 77 ext...

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