Qiagen QIAcuity 911000, Руководство пользователя

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QIAcuity User Manual 06/2022 221
1. Click the applicable wells in the plate layout. For more information, see Selecting wells for
analysis.
2. Select the applicable wild-type target from the Wild type target list.
3. Select the applicable edited target from the Edited target list.
4. To view the results of the analysis, click Show results .
5. The results are divided into several tabs. To view the contents of the tab, click the tab title.
List tab for genome editing
The List tab contains a table with an overview of the analyzed wells. The following columns are
available in the table:

Well ID – This column represents the well position in the plate layout.

Sample/NTC/Control – This column shows the sample, NTC, or control name with its
corresponding icon that identifies the sample or indicates whether the entry is an NTC or
control.

Reaction mix – This column contains the icon and the name of the well.

Target – This column shows all target names with its corresponding target type. Targets that
were selected as wild type or edit type are marked accordingly.

Concentration (copies/ul) – This column shows the concentration assigned to each target or
channel.

CI (95%) – This column shows the value of the confidence interval at a 95% confidence level.

Edited fraction – This column shows the edited fraction for the edited target as percentage.

CI (95%) – This column shows the value of the confidence interval for the edited target at a
95% confidence level.