Zeiss SIGMA Series, Instruction Manual

Page: 137 / 170

ZEISS 7 Troubleshooting | 7.1 Chamber
7.1.6 Replacing the Chamber Door Seal
Possible reasons for replacing the chamber door seal are the following:
§
Chamber door does not close tightly
§
Bad chamber vacuum
The procedure consists of the following steps:
§
Venting the Specimen Chamber [
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87]
§
Replacing the O-ring [
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137]
§ Evacuating the Specimen Chamber [
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89]
7.1.6.1 Replacing the O-ring
WARNING
Suffocation hazard due to lack of oxygen
Gaseous dry nitrogen is used to vent the specimen chamber during specimen exchange. Inhal-
ing nitrogen may cause unconsciousness.
4
During specimen exchange, keep the chamber door open as short as possible.
4
Do not inhale the air from within the specimen chamber.
4
Ensure that the area around the microscope is sufficiently vented.
4
If you begin to experience symptoms of asphyxia (for example: rapid breathing, loss of
mental alertness and/or muscular coordination, depression of sensations, emotional insta-
bility, fatigue) leave the room immediately and inform the facility’s safety officer.
CAUTION
Moving the specimen stage
Fingers can be trapped by the moving specimen stage.
4
Always close the chamber door before moving the specimen stage.
4
To remove parts fallen into or near to the stage use a tool (e.g. tweezers) instead of your
fingers.
CAUTION
Closing the chamber door
Fingers can be pinched when closing the chamber door.
4
Use the door handle to close the chamber door.
4
Ensure not to get your fingers caught in the chamber door gap.
NOTICE
Contamination caused by fingerprints
Contamination caused by fingerprints can lead to vacuum deterioration or prolonged pumping
times.
4
Always wear lint-free gloves when touching the specimen, specimen holder, or stage.
Instruction Manual ZEISS SIGMA series | en-US | Rev. 7 | 352102-9344-006 137