Zeiss SIGMA Series, Instruction Manual

Page: 102 / 170

5 Commissioning and First Operating Steps | 5.6 Finding Appropriate Detector Settings ZEISS
Fig. 66: An eledone tentacle
The following settings are recommended for the SE detector:
EHT Typical WD Collector Voltage
500 V – 5 kV 2–8 mm
§
Adjustable from −250 V to +400 V
§ Standard applications: +300 V
At a high magnification, you can opti-
mize the image by varying the collector
voltage.
§
Pseudo-backscattered (BSE) image:
−250 V to −50 V
This produces a topogaphic image of the
sample with no material contrast.
5 kV – 30 kV min. 6 mm
Procedure 1. In the SEM Controls panel, select the Detectors tab.
2. From the
Signal A drop-down list, select SE2 .
3. Adjust the EHT, working distance (WD), and collector voltage according to the suggestions
in the table in order to optimize the image.
Info
For detailed information on working with the SE detector refer to Acquiring an Image [
}
83] .
5.6.5 Setting up the VPSE Detector
The VPSE detector is designed for VP applications. The VP mode enables analyzing and imaging of
non-conducting, strongly gassing or moist specimens without any need for specimen preparation.
Fig. 67: A rosemary leaf.
102 Instruction Manual ZEISS SIGMA series | en-US | Rev. 7 | 352102-9344-006