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Axioskop 40
OPERATION
Axioskop 40 FL
Illumination and contrast procedures
Carl Zeiss
B 40-810 e 12/01
3-25
(3)
Setting transmitted-light phase
contrast on the Axioskop 40 and
Axioskop 40 FL
•
Swivel phase-contrast objective, e.g. designated
with Ph 1, into the beam path.
•
Insert phase stop labeled the same as the
phase-contrast objective, e.g. Ph 1, on the
turret disk of the universal condenser. When
using the 0.9/1.25 H condenser, place slider
into the right or left stop position
•
To check centering and congruence of the
bright phase stop (in the condenser) with the
dark phase ring (in the objective), remove one
eyepiece from the tube and replace it with the
auxiliary microscope. Use the correction device
on the auxiliary microscope to focus on the
phase stop and the phase ring in the objective
exit pupil.
•
If congruence is not perfect (3-21/
A
), use the
two SW 1.5 screwdrivers (3-20/
1
and
4
) on the
two centering screws (3-20/
2
and
3
) to recenter
the bright phase stop until complete
congruence with the dark phase ring is
achieved (3-21/
B
). With the 0.9/1.25 H
condenser, use the knurled screws on the slider
for your centering procedure (see Fig. 3-22/
1
).
•
Remove the auxiliary microscope from the tube
and re-insert eyepiece in the tube.
Normally, however, centering is not required, since
the phase stops are factory-centered and centering
is retained even if the universal condenser is
removed from the condenser carrier and attached
again.
To enhance the image contrast, an interference wide-band filter, green 32 x 4, can be placed on the
luminous-field diaphragm or inserted into the filter mount (where available).
Complete phase contrast is only achieved if the bright phase stop (in the condenser) and the dark phase
ring (in the objective) are exactly congruent in the illumination beam path (3-21/
B
).
Fig. 3-20
Centering phase stop on the
universal condenser
Fig. 3-21
Centering phase stop (light, in the
condenser) to the phase ring (dark,
in the objective)
Summary of Contents for Axioskop 40
Page 1: ...Operating Manual Axioskop 40 Axioskop 40 FL Routine microscope...
Page 14: ...INTRODUCTION Axioskop 40 Carl Zeiss Axioskop 40 FL 0 14 B 40 810 e 12 01...
Page 30: ...INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss Axioskop 40 FL 1 16 B 40 810 e 12 01...
Page 77: ...Axioskop 40 OPERATION Axioskop 40 FL Carl Zeiss B 40 810 e 12 01 3 3...
Page 128: ...OPERATION Axioskop 40 Carl Zeiss Axioskop 40 FL 3 54 B 40 810 e 12 01...
Page 146: ...APPENDIX Axioskop 40 Carl Zeiss Axioskop 40 FL A 8 B 40 810 e 12 01...