OPERATION
Axioskop 40
Carl Zeiss
Illumination and contrast procedures
Axioskop 40 FL
3-26
B 40-810 e 12/01
3.5.3
Setting transmitted-light VAREL contrast
(1)
General principle
VAREL contrast offers a relief-like and rich contrast display of specimen structures.
It can also be used for thick, transparent specimens that cannot be contrasted with phase contrast.
Using the segment-like, high aperture illuminated, absorbing VAREL ring in the objective the intensities
of direct and deflected light are balanced. This gives a richer contrast, relief-like image.
(2)
Transmitted-light dark field components for Axioskop 40 and Axioskop 40 FL
−
0.9/1.25 H condenser with slider, equipped with VAREL segment stops Var 1 / Var 2
−
Objectives suitable for VAREL segment stops: A-Plan 10x/0.25 Ph 1 Var 1 and/or A-Plan 40x/0.65 Ph 2
Var 2.
(3)
Setting transmitted-light VAREL
contrast on the Axioskop 40 and
Axioskop 40 FL
•
Completely open aperture diaphragm (3-22/
4
)
on the 0.9/1.25 H condenser (3-22/
3
).
•
Move VAREL segment stop into the beam path
and guide slider (3-22/
2
) to the right or left
stop position.
•
Swivel in appropriate VAREL objective on the
nosepiece.
•
Where necessary, center the VAREL segment
stops by adjusting the knurled screws (3-22/
1
)
in the same manner as for the phase stops
(cf. fig. 3-21).
Fig. 3-22
Condenser 0.9/1.25 H and slider
with VAREL segment stop
Summary of Contents for Axioskop 40
Page 1: ...Operating Manual Axioskop 40 Axioskop 40 FL Routine microscope...
Page 14: ...INTRODUCTION Axioskop 40 Carl Zeiss Axioskop 40 FL 0 14 B 40 810 e 12 01...
Page 30: ...INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss Axioskop 40 FL 1 16 B 40 810 e 12 01...
Page 77: ...Axioskop 40 OPERATION Axioskop 40 FL Carl Zeiss B 40 810 e 12 01 3 3...
Page 128: ...OPERATION Axioskop 40 Carl Zeiss Axioskop 40 FL 3 54 B 40 810 e 12 01...
Page 146: ...APPENDIX Axioskop 40 Carl Zeiss Axioskop 40 FL A 8 B 40 810 e 12 01...