![background image](http://html1.mh-extra.com/html/zeiss/axioskop-40/axioskop-40_operating-manual_3382221022.webp)
INSTRUMENT DESCRIPTION
Axioskop 40
Carl Zeiss
Objectives
Axioskop 40 FL
1-8
B 40-810 e 12/01
1.4
Objectives
The objectives are the optical heart of the
microscope. The following is an example of how
objectives can be labelled:
Achroplan 10x/0.25
∞
/0.17
where
10x
: objective magnification, with a defined
color ring on the objective being allocated
to each magnification step (Zeiss color
code)
0.25 : numerical aperture
∞
: infinite image distance; these objectives
can only be used with ICS microscopes
from Carl Zeiss
0.17 : can only be used with cover slip thickness
D = 0.17 mm
or
−
: can be used with cover slip thickness D = 0
or 0.17 mm
Other labels:
Oil
: oil immersion objective
Ph 2 : phase contrast objective with a green color ring and phase stop Ph 2
Color ring code for objective magnification:
Color ring on
objective
black
brown
red
orange
yellow
green
light blue dark blue white
Magnification
factor
1.25x
2.5x
4x; 5x
6.3x
10x
16x;20x;
25x;32x
40x; 50x
63x
100x;
150x
Objective magnification multiplied by eyepiece magnification (usually 10x) results in overall visual
magnification: e.g. 10 x 10 = 100x.
When working with the microscopes, total magnification should not drop below or exceed the range of
useful magnification. The range of useful magnification was defined by Ernst ABBE as 500 to 1,000
times the numerical aperture of the objective used . There is no resolution for further details above that
limit. Accordingly, the range of useful magnification for an objective with a numerical aperture of 0.25 is
between 125x and 250x.
Exact observance of the cover slip thickness of 0.17 mm is all the more necessary the higher the
numerical aperture of the objective. For this reason, certain objectives can be set to different cover slip
thicknesses (due to correction mount). To this end, a specimen area is searched for, and the position of
the correction ring where optimum focus and image contrast are obtained is determined (refocusing is
invariably required).
Fig. 1-1
Objective
Summary of Contents for Axioskop 40
Page 1: ...Operating Manual Axioskop 40 Axioskop 40 FL Routine microscope...
Page 14: ...INTRODUCTION Axioskop 40 Carl Zeiss Axioskop 40 FL 0 14 B 40 810 e 12 01...
Page 30: ...INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss Axioskop 40 FL 1 16 B 40 810 e 12 01...
Page 77: ...Axioskop 40 OPERATION Axioskop 40 FL Carl Zeiss B 40 810 e 12 01 3 3...
Page 128: ...OPERATION Axioskop 40 Carl Zeiss Axioskop 40 FL 3 54 B 40 810 e 12 01...
Page 146: ...APPENDIX Axioskop 40 Carl Zeiss Axioskop 40 FL A 8 B 40 810 e 12 01...