PerkinElmer AVIO 500, Customer Hardware And Service Manual

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Automatically Aligning the Torch Viewing Position
To perform UV channel wavelength calibration:
1. Aspirate the Wave Cal solution (Part No. N0582152).
2. Select UV , then click on Wavelength Calibration in the Spectrometer Control
window.
The UV calibration may take several minutes. You may view the spectra used
for calibration by clicking on Spectra in the toolbar. When the UV calibration
is complete, the UV coefficients and RMS (Root Means Square) value will be
displayed in the Wavelength/Calibration window. These values should meet
the following specifications:
If these specifications are not met, repeat the wavelength calibration and make sure
all peaks can be seen in the Spectra Display window. If these specifications are still
not met, a complete optical alignment may be required. Contact a PerkinElmer
Service Representative.
To perform VIS channel wavelength calibration:
1. Aspirate VIS Wavecal mix (Part No. N9302946).
2. Select VIS , then click on Wavelength Calibration in the Spectrometer
Control window.
The VIS calibration may take several minutes. You may view the spectra used for
calibration by clicking on
Spectra in the toolbar. When the VIS calibration is
Note
The spectrometer will automatically change to radial viewing to prevent
saturation of the detector.
UV Coefficients and RMS Specification
First Coefficient
absolute value < 2.0
Second Coefficient absolute value < 2.0
Third Coefficient absolute value < 8.0
RMS
(measures variability of
wavelength offsets) < 2.0