HANSATECH FMS1, Manual

Page: 54 / 86

54 FMS 1 - Fluorescence Monitoring System
© 2017 Hansatech Instruments Ltd
following command line should be added:
ACT: 10
The fluorescence signal normally passes through a series of changes immediately after
illumination whilst the photosynthetic processes become light adapted. Measurement is often
delayed until the sample has reached “steady state”. The scripting language contains several
“Wait” commands which are suitable in different contexts e.g. waiting for a key-press from the
user, waiting for a defined period of time or waiting for a digital input from some external
apparatus ( Hansatech Scripting Language ). In this example the “wait for a fixed period of time”
command will be used by “dragging” the clock icon from page 1 to the script (Figure 2-32); the
program will prompt for the wait duration in units of seconds.
Fig. 2-32 . Wait for fixed period Icon.
A value of 100 sec should be entered to generate the following command line.
WAIT: 100.0
After 100 sec the next command will be executed. This will be a measurement of the quantum
efficiency of PSII (fPSII) which is available from page 2 of the icons. The control functions required
for fPSII measurement are similar to those of Fv/Fm. Hence the same types of parameters are
used. Although it should be noted different values for any parameter may be used. If default
parameters are used the following command line should be inserted:
f
PSII : 2.5, 100, 0.7
The final script should appear similar to that in figure 2-33.