GE AKTA avant, User Manual

Page: 374 / 538

Possible cause and action Problem
•
Bad pump function
Check that the pump is operating properly. See Exam-
ples of pump pressure curves, on page 400 for example
of pump pressure curves.
•
Air in the flow path
Purge the pumps. See Section 5.4.1 Prime buffer inlets
and purge system pumps, on page166 and Section 5.4.3
Prime Q inlets, on page 178.
•
Poor mixing function
Check that the correct Mixer chamber size is used.
See Select Mixer chamber, on page 157 for recommen-
dations. To change the Mixer, see Section 7.8.2 Replace
the Mixer, on page 338.
Check the motor operation of the Mixer. Place a
magnet close to the mixer chamber during run. The
magnet should vibrate.
Check that the Mixer chamber is free from solids. To
open the mixer, see Section 7.3.2 Replace the inline
filter, on page 275.
Waves on the gradient
•
A charged particle has been detected
Prepare the sample so that charged particles are
eliminated.
•
Air bubbles are passing through the flow cell
Check for loose tubing connections.
Use the Flow restrictor.
Ghost peaks appear in
the gradient profile
•
Dirt in the tubing
Make sure that the tubing has been washed properly.
•
Bad pump function
Make sure that the pump operates properly. See Ex-
amples of pump pressure curves, on page400 for exam-
ple of pump pressure curves.
•
The Mixer chamber is too large
Change to a Mixer chamber with a smaller volume.
See Select Mixer chamber, on page 157 for recommen-
dations. To change the Mixer, see Section 7.8.2 Replace
the Mixer, on page 338.
Non-linear gradients or
slow response to %B
changes
Note:
Remember, conductivity
at high salt concentra-
tions are intrinsically not
linear.
374 ÄKTA avant User Manual 29035184 AE
8 Troubleshooting
8.3 Troubleshooting: Monitors