GE AKTA avant, User Manual

Page: 72 / 538

Description Port
Port for connection of a loop or a Loop valve. Used to empty a loop into
the flow path. LoopE
Loop and System pump waste W1
Sample flow waste W2
1
For larger sample volumes connect the syringe with the help of a union Luer female to 1/16 ″
male (default). For smaller volumes it it recommended to use the Fill port INV-907 (18-1127-66)
and connect a syringe with needle.
Description Flow path
The system flow is directed onto the column or column
valve. Sample can be manually injected into the loop.
Excess sample leaves the valve through waste port W1. Manual load - Default
position of the valve
The system flow is directed through the loop and onto the
column or column valve. If the sample pump is used, the
flow entering the SaP port is directed to waste port W2. Inject
The system flow is directed to waste port W1. If the sample
pump is used, the flow entering the SaP port is directed
to the column valve. System pump waste
The flow entering the SaP port is directed to the column
valve. This position is used with the sample pump. Flow
entering the SyP port is directed to waste port W1. Direct inject
The flow entering the SaP port is directed to the loop. This
position is used with the sample pump. Excess sample
leaves the valve through waste port W1. The flow entering
the SyP port is directed to the column or the column valve. Sample pump load
The flow entering the SaP port is directed to waste port
W2. This position is used with the sample pump. The flow
entering the SyP port is directed to the column via the
loop. Sample pump waste
Note: •
In order to avoid sample carry-over when switching techniques for loading
samples, wash the Injection valve with buffer between the loading of two
different samples. For example, when switching from loading sample onto
the loop to loading sample directly onto the Column with the valve in Direct
inject position.
•
Make sure that the SaP port is plugged with a stop plug if the Sample
Pump is not connected.
72 ÄKTA avant User Manual 29035184 AE
3 Standard instrument modules
3.5 Injection valve