GE AKTA avant, User Manual

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9.6 Predefined methods and phases
Introduction
A predefined method contains a set of phases, each phase reflecting a specific stage of
a chromatography or maintenance run. You can select additional phases from the phase
libraries and add these to an existing method, or remove phases that are not required.
The predefined purification methods have default values with suitable running conditions
for the chosen column type such as flow and pressure limits. Other settings (e.g., sample
application technique, sample volume, elution profile and fractionation) are set on the
Phase Properties pane in the appropriate phases.
Predefined purification methods
The Method Editor has predefined methods for different separation techniques. The
methods include a number of relevant phases.
The following table describes the available predefined purification methods and which
phases that are included.
Included phases Principle Predefined purification
method
After equilibration and sample application,
the protein of interest is adsorbed to the
column ligand. After a wash to remove
unbound sample, elution is performed ei-
ther by using a buffer containing a competi-
tor to displace the protein of interest, or by
changing the pH or ionic strength. After
column re-equilibration, a fraction collector
accumulator wash is done to minimize the
risk for salt precipitation. Affinity Chromatography
(AC)
ÄKTA avant User Manual 29035184 AE 449
9 Reference information
9.6 Predefined methods and phases