GE AKTA avant, User Manual

Page: 167 / 538

Action Step
In the Process Picture: 3
•
Click the inlet valve icons. (Click both the Inlet A and Inlet B icons, one
at a time, if both inlets are to be primed.)
•
Click the position of the inlet to be filled. Fill the positions in reverse al-
phabetical order and start with the highest number. For example, if all
the seven inlets in Inlet Valve B are to be filled, click them in the following
order: B7, B6 . . . B1, assuming that B1 is the starting buffer.
Result: The inlet valve switches to the selected port.
Connect a 25 to 30 ml syringe to the purge valve of one of the pump heads
of System Pump B. Make sure that the syringe fits tightly into the purge
connector. 4
Open the purge valve by turning it counter-clockwise about three quarters
of a turn. Draw liquid slowly into the syringe until the liquid reaches the
pump. 5
Close the purge valve by turning it clockwise. Disconnect the syringe and
discard its contents. 6
Repeat steps 3 to 6 for each piece of inlet tubing that is to be used during
the run. In the final inlet position, draw liquid into the syringe through both
purge valves. 7
ÄKTA avant User Manual 29035184 AE 167
5 Operation
5.4 Prime inlets and purge pump heads
5.4.1 Prime buffer inlets and purge system pumps