GE AKTA avant, User Manual

Page: 68 / 538

Quaternary gradients
The Quaternary valve can be used to create a gradient using four different solutions si-
multaneously in any combination. The percentage of each solution is controlled by in-
structions in the method. It is possible to form gradients that changes the percentage
of two, three or four solutions linearly over time. This is useful when advanced methods
are developed.
In ÄKTA avant 25 Quaternary gradients can be created at flow rates up to 25
ml/min. In ÄKTA avant 150 Quaternary gradients can be created at flow rates
up to 40 ml/min. Note:
Function of Sample inlet valve
The Sample inlet valve enables automatic loading of different samples when using the
Sample pump to inject sample directly onto the column or to fill a sample loop.
The Sample inlet valve has an inlet dedicated for buffer. This Buff inlet is used in methods
to fill the Sample pump with solution before sample is introduced. The Buff inlet is also
used to wash the Sample pump with buffer between runs.
The air sensor integrated in the Sample inlet valve is used when sample is applied from
a vessel onto a column by selecting Inject all sample using air sensor in the Sample
application phase of a method. This function uses the Buff inlet to finalize sample injection
and to remove air from the Sample pump.
68 ÄKTA avant User Manual 29035184 AE
3 Standard instrument modules
3.4 Inlet valves