GE AKTA avant, User Manual

Page: 328 / 538

Conductivity Monitor - user
calibration
Follow the instruction to calibrate the conductivity flow cell constant.
Action Step
Make sure that the instrument has been switched on for at least one hour. 1
In the System Control module, on the System menu, click Settings. 2
Result: The System Settings dialog box opens.
In the System Settings dialog, select Conductivity:Cond temp compensation.
Set the Compensation factor to 0, and click OK. 3
In the Calibration dialog box, in the Monitor to calibrate list, click Conduc-
tivity monitor - user calibration. 4
Prepare at least 25 ml of calibration solution. Use either a 0.1 M KCl certified
conductivity standard solution, or accurately prepare your own 1.00 M NaCl
solution. 5
Immerse a piece of sample inlet tubing, for example S1, in the calibration
solution. 6
Open the System Control module. On the Manual menu, click Execute
Manual Instructions. 7
Result: The Manual instructions dialog box opens.
In the Manual instructions dialog box: 8
•
Select Flow path:Injection valve, in the Position list, click Direct inject.
Click Insert.
•
Select Flow path:Column position, in the Position list, click By-pass.
Click Insert.
•
Select Flow path:pH valve and set the Restrictor to In-line and the pH
to Off-line. Click Insert.
•
Select Flow path:Sample inlet, select a position in the Position list (in
this example S1). Click Insert.
•
Select Pumps and Pressures:Sample flow and enter 1.0 to ml/min in
the Flow rate box. Click Insert.
•
Click Execute.
Result: Calibration solution is pumped through the system by the Sample
pump.
Pump in at least 25 ml of the calibration solution, and wait until the conduc-
tivity signal is stable. 9
328 ÄKTA avant User Manual 29035184 AE
7 Maintenance
7.7 Calibration procedures
7.7.3 Calibrate the Conductivity Monitor