GE AKTA avant, User Manual

Page: 297 / 538

7.6.3 Perform Column CIP
Introduction
The Column CIP method is used to clean the column after purification runs, to remove
non-specifically bound proteins and to minimize the risk for carry-over between different
purification runs.
WARNING
• Hazardous substances. When using hazardous chemicals,
take all suitable protective measures, such as wearing protec-
tive clothing, glasses and gloves resistant to the substances
used. Follow local and/or national regulations for safe opera-
tion and maintenance of the product.
•
Hazardous chemicals during run. When using hazardous
chemicals, run System CIP and Column CIP to flush the entire
system tubing with distilled water, before service and mainte-
nance.
If hazardous chemicals are used for system or column cleaning, wash the
system or columns with a neutral solution in the last phase or step.
Tip:
Maintenance interval
Perform a Column cleaning in place (Column CIP) when required, for example between
runs where different samples are used.
Required material
The following materials are required:
•
Appropriate cleaning solutions. Please refer to the instructions for use of the column.
ÄKTA avant User Manual 29035184 AE 297
7 Maintenance
7.6 Maintenance when required
7.6.3 Perform Column CIP