GE AKTA avant, User Manual

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1.2 ÄKTA avant overview
Introduction
ÄKTA avant is a preparative liquid chromatography system intended for purification of
proteins as well as other bio-molecules and used for method and process development.
The system is equipped with valves, detectors and fraction collector, integrated in the
flow path, needed for automated control and optimal performance. The system can be
used to screen for columns, media, and running parameters to purify selected proteins
This section gives an overview of the ÄKTA avant instrument and UNICORN software.
For detailed information about UNICORN, see the UNICORN manuals listed in User doc-
umentation on the CD, on page 12. For detailed information about the instrument, see
Chapter 2 The ÄKTA avant instrument, on page 15.
Main features
The main features of ÄKTA avant are listed below.
•
The ÄKTA avant is a complete system including all modules needed for normal method
and process development.
•
The ÄKTA avant system can be easily extended with additional modules, e.g. for extra
samples, buffers, outlets, columns and integration of optional components.
•
The ÄKTA avant instrument is compact with a built-in fraction collector.
•
Cooling of the built-in fraction collector enables secure storage of the purified samples
and unattended runs over night.
•
The built-in Instrument display shows instrument status and method state.
•
ÄKTA avant is controlled by the UNICORN software: a complete package for control,
experimental planning, supervision and evaluation of chromatography instruments
and purification runs.
-
Purification and maintenance methods are easily created using predefined
methods and method phases. Method phases are displayed graphically in a
method outline and phase properties, which makes methods and phases easy
to handle and edit.
-
Run statistics history for individual columns can be saved (e.g., total number of
runs and number of runs since last cleaning procedure).
-
BufferPro (automatic buffer preparation) facilitates screening for optimal buffer
compositions as well as preparation of single buffers.
- Design of Experiments (DoE) is an integrated part of UNICORN that provides an
organized approach that connects experiments in a rational manner.
10 ÄKTA avant User Manual 29035184 AE
1 Introduction
1.2 ÄKTA avant overview