GE AKTA avant, User Manual

Page: 192 / 538

Then... When preparing to
inject...
•
in the Method editor, make the following selections
for the Sample Application phase of the method to
be run:
-
select Inject sample directly onto column, and
-
select Inject all sample using air sensor.
•
manually prime the sample inlet tubing with sample
or buffer, see Prime sample inlets, on page 173, and
make sure that the tubing is immersed in sample be-
fore starting the run.
•
make sure that the flow path from the sample inlet
valve up to the injection valve is filled with sample or
an appropriate buffer and that the buffer inlet tubing
is immersed in buffer.
all the sample
192 ÄKTA avant User Manual 29035184 AE
5 Operation
5.7 Sample application
5.7.1 Sample application using direct injection into the column