Fluid Imaging
Operating Principles
Rev. D
Dimension 3100 Manual
163
Note:
The difference between the vertical deflection before engaging and the setpoint
is related to the amount of force that the cantilever probe tip applies to the
sample.
Note:
Verify that there is not too large a difference between the setpoint and the
vertical deflection signal before engaging. Samples are typically softer in liquid
than in air. It may be desirable to reduce the setpoint once engaged to obtain the
minimum tracking force.
Adjust Photodetector
1. Adjust the photodetector so that the red dot moves toward the center of the laser alignment
window using the two photodetector adjustment knobs located on the side of the Dimension
head.
2. Set the
Vertical deflection
to
0
.
3. Verify that the red dot is centered and elliptical in shape in the laser alignment window.
10.5.12 Locate Tip
1. Using the mouse, select
Locate Tip
from under the
Stage
pop-down menu or click the
Locate Tip
icon.
2. Center the tip on the cantilever under the cross hairs using the two adjustment screws located
to the left of the optical objective on the microscope.
3. Focus on the tip end of the cantilever using the trackball while holding the bottom left button.
Note:
See
for more detailed instructions for locating the tip.
10.5.13 Focus Surface
When focusing on the sample surface in
air
before adding fluid to the sample area, the following
procedure is necessary:
1. Align laser
2. Locate tip
3. Focus surface
4. Add fluid
5. Readjust laser alignment
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