background image

 

 

LM

A.

 

Sy

1.

 

Si

2.

 

Tu

3.

 

Tu

Xe

4.

 

St

 

B.

 

Ha

1.

 

Lig

2.

 

Se

3.

 

Ha

lig

4.

 

Pr

cl
kn
Th

bu

MW

 

Microscop

ystem

 

Start

u

gn

 

into

 

the

 

lo

urn

 

on

 

(or

 

wa

urn

 

on

 

the

 

mi

enon

 

lamp

f

 

tart

 

cellSens

 

D

ardware

 

Cont

ght

 

path

 

bars

elect

 

an

 

objec

alogen

 

lamp

 

ght

 

will

 

be

 

vis

rior

 

Z

stage

 

C

ose

 

to

 

the

 

ob

nob;

 

the

 

exte

he

 

selected

 

sp

utton

 

on

 

the

 

c

 

e

 

j

 

py

 

Laboratory

   Olym

up.

                

og

 

book

 

on

 

th

ake

 

up)

 

the

 

co

icroscope

 

b

,

(only

 

if

 

you

 

n

Dimensio

n

trol

 

and

 

Setti

s

g

 

directs

 

lig

ctive

 

lens

 

by

 

t

dial

 

knob

 

i

c

sible

 

from

 

the

Control

 

Box

d

bjective

 

and

 

D

nt

 

of

 

the

 

fine

peed

 

appears

controller

.)

 

d

 

y

 

mpus BX

he

 

desk

 

(start

omputer

 

and

 

 

the

 

main

 

con

need

 

it

 

for

 

flu

 

program.

 

ing.

 

ght

 

to

 

eyepiec

turning

 

the

 

no

controls

 

the

 

b

e

 

condenser

 

le

moves

 

the

 

s

DOWN

 

button

e

 

movement

 

c

s

 

on

 

the

 

LED

 

s

X53 Digit

t/end

 

time).

select

 

“USER”

ntrol

 

unit

c

,

 

t

orescence

 

im

ce,

 

camera,

 

o

osepiece

 

turr

brightness

 

of

 

ens.

  

stage

 

up

 

or

 

d

n

 

brings

 

it

 

dow

can

 

be

 

selecte

screen

 

(

Note:

f

 

tal Micro

 

and

 

enter

 

th

the

 

Prior

 

rem

maging).

 

or

 

both.

   

ret

h

 

by

 

hand

transmission

own

 

to

 

focus

wn

 

quickly.

  

F

ed

 

High

 

(H),

 

M

:

 

DON’T

 

USE

 

t

b

c

 

O l y

oscope 

he

 

password

 

mote

 

z

stage

 

c

d.

  

n

 

light

 

for

 

brig

s

 

on

 

specimen

Finer

 

moveme

Medium

 

(M),

 

the

 

focus

 

kno

h

 

b

 

i

 

m p u s

 

B X

User Gu

on

 

the

 

log

in

controller

d

,

 

ght

 

field

 

view

n.

 

The

 

UP

 

but

ent

 

of

 

stage

 

c

or

 

low

 

(L)

 

by

 

ob

 

on

 

the

 

mic

c

 

X 5 3

 

U s e r

uide 

n

 

window.

 

DP26

 

color

 

ca

ing.

  

The

 

tran

tton

 

brings

 

th

can

 

be

 

contro

pressing

 

“SP

croscope

 

and

g

 

 

G u i d e

|

 

1

amera

e

,

 

and

nsmission

 

he

 

stage

 

up

 

olled

 

with

 

the

 

EED”

 

button.

d

 

the

 

ZERO

 

g

1

 

d

 

g

 

Summary of Contents for BX53

Page 1: ...epiec turning the no controls the b e condenser le d moves the s DOWN button e movement c s on the LED s X53 Digit t end time select USER ntrol unitc t orescence im ce camera o osepiece turr brightness of ens stage up or d n brings it dow can be selecte screen Note f tal Micro and enter th the Prior rem maging or both reth by hand transmission own to focus wn quickly F ed High H M DON T USE t b c ...

Page 2: ...tage election of im irror cube for t shutter ic image acqu he program f orescence ima x objective by w will pop up n the messag ns on the Obs e with Prior z maging mode r different co uisition or your imag aging for fluor y pressing an Turn the obj ge window It servation Me stage contro O l y i e BF or Flu lors of fluore ing needs i e rescently labe objective jective nosep t is critical to m thods...

Page 3: ...uisition era is ON and is pushed in and obtain p ith Spot 30 Click the but ctory click Sn king the right XM10 mono tic exposure de list You can choos selected from and the lowe proper exposu region and a tton again to nap t mouse butto ochrome cam to obtain bet se a lower res m the camera er bar11 is p ure and white utomatic wh stop live view button and s on over the im O l y mera for fluor tter i...

Page 4: ...emote co utter buttonc rescence mirr ods buttons e light path b and XM10 cam b ontroller box t c on the rem ror cube by p barsf are in mera is select to use mercu mote box ressing Mirro excep correct posit ted O l y ry arc lamp li or buttonsD pt BF and DIC tion the uppe e m p u s B X ight for fluore in the contro C The select er bar at the X 5 3 U s e r escence The oller box or cl ed mirror wil mi...

Page 5: ... tiff files Se bit image Us ultiple channe images for ea th View Scale tamp comma image as JPE e acquisition w eck the Auto en and TxRe o show curren ot 30 is use ditions are set the Automat n to read the ed click the ulting multich er as soon as t at without an elect Image M se File Save a els Or use Fil ach channel e bar comma and These an EG JFIF jpg with an exam matic Proces button d red chann...

Page 6: ... uplicate if it i dow tab ast of the disp ram or the Di oose the cha ick the color c st the histogr is necessary t from play can be ad splay Enhanc nnels to be v channels you ram and the b O l y to make any c m the main wi djusted cement appli visible eye sy want to show brightness co m p u s B X changes in th indow tabs in es to all the v mbol indi w and click o ontrast of the X 5 3 U s e r e ima...

Page 7: ...l im e image type f From the Ed peration will g Use Edit Cop cale bar in pix sion Selector channel imag d click OK mage you just from 16 bit g it menu use generate a co py Display to xel unit losin r Use Image ges Then use O l y t made as abo grayscale to 8 Edit Select A mposite imag o Clipboard an g some dime Separate Ch e Image Com m p u s B X ove there ar bit color All then Edit ge with the sa ...

Page 8: ...image me from a z st ame view and enu range of fram w starting pos se a new end ake sure all th n and select E ame will be ge Objective s acquired at el To set the ntroller or by the stage to fo buttonc wh er region close Image acquis end a step siz this step size accordingly es You can co can be manip ack by clickin d selecting Ex mes within the sition within t position and e channels ar Extract ...

Page 9: ...ed y ack frames int aximum Proje you can displ y cross hairlin ptions d x y slice pos to a single 2D ection Over Z ay a cross sec e to the desir sition D image by sel on the naviga ctional z dime red position such as O l y lecting Proces ator bar men ension view o Extended Slic m p u s B X ss Projection u of an x and y ce X 5 3 U s e r ns Maximum plane You ca you can visu G u i d e 9 m Z command a...

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