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Instructions

Notes

This instruction manual is for the Olympus culture microscope model CKX53.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of the 
microscope, we recommend that you study this manual thoroughly before operating the microscope, 
and always keep this manual at hand when operating the microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
For details of products included in the configuration of this system, see page 7.

CKX53

A X 8 6 6 0

Culture Microscope

Optical microscope and accessory

Summary of Contents for CKX53

Page 1: ...pe we recommend that you study this manual thoroughly before operating the microscope and always keep this manual at hand when operating the microscope Retain this instruction manual in an easily acce...

Page 2: ...designed to provide reasonable protection against harmful interference when the product is operated in a commercial environment This product generates uses and can radiate radio frequency energy and i...

Page 3: ...on the stage 11 2 When using the optional stage unit 11 3 When using the high container Procedures to attach detach the condenser portion 12 3 3 Observation methods Bright field observation Phase con...

Page 4: ...ocusing on the specimen 16 9 Moving the specimen 16 3 5 Recording with the camera 17 1 Changing between the visual light path and the camera light path 17 2 Focusing on the specimen 17 3 Acquiring the...

Page 5: ...ption 22 4 9 Operating procedures of CKX3 SLPIC option 23 4 10 Selecting procedures of the specimen holder option 23 4 11 Selecting procedures of the fluorescence mirror unit including options 24 1 Se...

Page 6: ...t Phase contrast standard set 40 7 7 Attaching the filter slider for transmitted light illumination 41 7 8 Attaching the light source for reflected light illumination Fluorescence set 41 7 9 Attaching...

Page 7: ...ation of living cells or of specimen taken from tissues to gain physiological or morphological information at hospitals or laboratories Typical field of applications are genetics human blood and tissu...

Page 8: ...loves etc or clean the product before operation Follow the procedures described in chapter Handling Precautions Follow the procedures described in chapter Handling Precautions page 5 prior to using th...

Page 9: ...ct or from the power outlet Install the product at a location where you can reach the power cord connector or the power outlet at hand to remove the power cord quickly This product complies with the e...

Page 10: ...bols CAUTION Mercury lamp housing Keep the AC adapter the power cord and other cables well away from the mercury lamp housing If the AC adapter the power cord and other cables contact a hot area of th...

Page 11: ...ay be damaged if excessive force is applied Never disassemble any part of the product Otherwise failure could be caused NOTE NOTE NOTE Handling Precautions Maintenance and Storage Maintenance 1 Do not...

Page 12: ...t until the lamp housing is cooled down sufficiently and keep it covered with a dust cover during storage 2 Before disposing of this product be sure to follow the regulations and rules of your local g...

Page 13: ...jective For details see page 39 Phase contrast slider CKX3 SLP IX2 SL Fluorescence illuminator CKX3 RFA Reflected light illumination unit U HGLGPS U LLG150 300 U LLGAD Camera DP22 DP73 Camera adapter...

Page 14: ...luded in the Bright field set Not included in the Bright field set and Phase contrast entry set Diopter adjustment ring a Stage center plate Brightness adjustment knob Main switch 1 Aperture iris diap...

Page 15: ...plate 2 Field iris diaphragm centering knob Field iris diaphragm lever 8 Filter slider for reflected light illumination 5 Burner centering knobs Mirror focusing screw Back of lamp housing Camera Adapt...

Page 16: ...hase contrast entry set and Phase contrast standard set you can move the microscope by raising the base portion front a and sliding the back side c of the microscope frame CKX53S 1 2 3 NOTE TIP 2 Atta...

Page 17: ...en holder and mechanical stage CKX3 MVR Place the container on the specimen holder option For containers and specimen holders corresponding to containers see page 23 Place the specimen holder option o...

Page 18: ...e brightness not so dazzling 1 TIP 2 2 Changing between bright field observation and phase contrast observation Phase contrast entry set Phase contrast standard set Fluorescence set When performing th...

Page 19: ...tion depending on containers or specimens 1 TIP 4 Selecting the objective Rotate the revolving nosepiece a to engage the objective b with the magnification to be used for observation in the light path...

Page 20: ...of the brightness adjustment knob Rotating the brightness adjustment knob in the front direction arrow direction makes the brightness brighter and rotating it in the opposite direction makes it darke...

Page 21: ...cury vapor has cooled down and liquefied Turn ON the main switch after 10 minutes For details refer to the instruction manual for each power supply unit The discharge type mercury burner may not be ig...

Page 22: ...e filter slider for reflected light illumination and engage the empty hole of the slider in the light path To adjust the brightness hold and slide the knob a of the filter slider for reflected light i...

Page 23: ...e following illustrations show the image acquisition areas of camera adapters compared with observation area by the eyepieces field number 22 1 TIP Refer to page 14 If the focusing of the image throug...

Page 24: ...mirror unit from attaching dust 1 1 2 2 TIP 2 Turning OFF the light source mercury burner Fluorescence set Turn OFF the main switch of the power supply unit For details refer to instruction manuals f...

Page 25: ...nd rotate coarse fine focusing knobs to bring the specimen into focus Change to the low magnification objective e g 10X While looking into the right eyepiece with your right eye rotate the right diopt...

Page 26: ...st the space according to the size of the container to be placed on the stage if necessary Loosen the umbra shield knob a Then move the umbra shield along with the umbra shield column to adjust the po...

Page 27: ...f the objective with correction collar the objective cannot exhibit its performance When using the objective with correction collar adjust the correction collar Objective with correction collar LUCPLF...

Page 28: ...ontrast slider see page 40 Rotate the knurled portion of the centering telescope to focus on the phase contrast ring slit image b Insert the centering knobs d to the centering screw holes 2 positions...

Page 29: ...the objective PLCN10X or CACHN10XIPC in the light path 1 TIP 2 c d 4 10 Selecting procedures of the specimen holder option The container size differs depending on each container maker For the size of...

Page 30: ...contains the B excitation fluorescence mirror unit and the G excitation fluorescence mirror unit as a standard mirror unit In addition to the B excitation or the G excitation fluorescence mirror units...

Page 31: ...hase contrast slider in the light path 12 The umbra shield ambient light shielding plate is engaged in the light path Remove the umbra shield from the light path 15 3 The image is poor The contrast of...

Page 32: ...Eliminate the fog The objective or the filter is dirty Clean the objective or the filter 5 The field iris diaphragm is not adjusted appropriately Adjust the field iris diaphragm until its image circu...

Page 33: ...large aperture 5 The mechanical stage CKX3 MVR does not work smoothly The fixing knob is loosened Tighten the fixing knob firmly 38 The specimen is heavy Place the light specimen on the stage and con...

Page 34: ...ary distance adjustment range 48 mm 75 mm Eyepiece tilt angle 45 Light path split ratio Visual light path 0 Camera light path 100 Visual light path 100 Camera light path 0 Camera port Olympus camera a...

Page 35: ...ptions iPC aperture common in 4X 10X 20X and 40X Empty hole Options 45 mm 2 pieces Fluorescence illuminator CKX3 RFA Fluorescence mirror unit slider Number of selectable positions 3 positions Applicab...

Page 36: ...LN20XPH Applicable slider IX2 SL PH2 phase contrast ring IX2 SLPH2 Applicable objective LUCPLFLN40XPH Applicable slider IX2 SL Specimen holder Dimensions 128 mm x 86 mm Dimension of container holding...

Page 37: ...icable burner SHI 130OL Burner capacity 130 W Average burner service life 2000 hours Rated input 100 240 VAC 50 60 Hz 2 0 A Liquid light guide U LLG150 length 1 5 m U LLG300 length 3 m Light guide ada...

Page 38: ...Stage center plate Objective Filter for transmitted light illumination Filter holder for transmitted light illumination Optical element ring Fluorescence mirror unit Filter slider for reflected light...

Page 39: ...ibed on the glass stage plate are readable from the top 1 TIP 1 Attaching the condenser portion Loosen the condenser fixing knob a to the extent that it does not fall off Hold the condenser diagonally...

Page 40: ...the side of the fluorescence mirror unit and push it in the arrow direction until the fluorescence mirror unit touches the end In this case attach the fluorescence mirror unit at 2 positions as shown...

Page 41: ...r clamping screws 2 positions a of the microscope frame using the Allen wrench and remove the dust cover b 1 2 3 4 Loosen the setting screws 2 positions c of the illuminator using the Allen wrench and...

Page 42: ...crew the adjuster portion l of support pillar for fluorescence illuminator into the screw hole of the column portion i until the screw becomes slightly visible 9 10 11 12 After the light source side p...

Page 43: ...ated If you want to attach the removed attenuator filter c back to the previous position be sure to place the at tenuator filter c on the attenuator filter which was used alone At this time face the s...

Page 44: ...he umbra shield column to adjust the height Decide the height and tighten the umbra shield knob a firmly The lower the position of the umbra shield is the more the ambient light can be blocked Adjust...

Page 45: ...a c b 1 For objectives provided with each set see page 29 For specifications of objectives including options see page 30 It is recommended to attach the objectives as shown in the picture where the r...

Page 46: ...set Phase contrast standard set 3 Attaching the phase contrast ring option In case of the centering phase contrast slider IX2 SL the phase contrast ring to be used for the phase contrast observation d...

Page 47: ...he burner clamping screws c 2 pcs Attach the positive pole of the specified mercury burner d to the fixed mount on the upper side then the negative pole to the mount on the lower side Be sure to use t...

Page 48: ...of the power supply unit to 0 0 For details refer to the instruction manual for the power supply unit Burner service life CAUTION When removing the mercury burner follow the procedures opposite to tho...

Page 49: ...paper surface to light TIP 1 2 3 4 4 Centering the mercury burner only when combined with the mercury lamp housing U LH100HG The mercury burner emits the light by means of discharge produced when a c...

Page 50: ...eft half of the field B in the picture Insert the Allen screwdriver in the mirror focusing screw on the rear of the mercury lamp housing and turn it to bring the mirror arc image e into focus C in the...

Page 51: ...nd put the fixing knob through the hole of the cable holder Then attach clamping knobs to each stage again and attach each stage to the plain stage For procedures to attach the stage to the plain stag...

Page 52: ...nty period are paid services If you have any questions please contact Olympus Check results Date Check items 1 More than 8 years have passed since original purchase of the illumination device or the t...

Page 53: ...ing portions indicate operating portions described in 2 1 Bright field set Phase contrast entry set Phase contrast standard set page 8 TIP Press the main switch of the microscope frame Place the speci...

Page 54: ...rescence observation procedures 9X axis knob page 16 aY axis knob page 16 page 47 2Umbra shield page 15 Place the specimen on the stage Engage the fluorescence mirror unit suitable for the specimen in...

Page 55: ...r cord which is approved by one of the agencies mentioned in Table 1 please use replacements approved by any other equivalent and authorized agencies in your country Country Agency Certification mark...

Page 56: ...Cables BASEC BASEC HAR 10 10 30 N V KEMA KEMA KEUR HAR 10 30 30 SEMKO AB Svenska Elektriska Materielkontrollanstalter SEMKO HAR 10 10 50 sterreichischer Verband f r Elektrotechnik VE VE HAR 30 10 50...

Page 57: ...Memo...

Page 58: ...Memo...

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Page 60: ...ower 446 Bongeunsa ro Gangnam gu Seoul 06153 Korea Distributed by Manufactured by 48 Woerd Avenue Waltham MA 02453 U S A Shinjuku Monolith 2 3 1 Nishi Shinjuku Shinjuku ku Tokyo 163 0914 Japan www oly...

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