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Sakura Tissue-Tek Polar DM, Руководство по эксплуатации

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Sakura Tissue-Tek Polar DM Скачать руководство пользователя страница 78

 

2-9

 

Preparing for Sectioning 

Adjusting the Lateral Movement of the Blade Holder   

You can slide the blade holder to the left and right.   
This way, the set disposable blade can be used fully.   
 
1.  Tilt toward you the blade holder left/right lever indicated in yellow 

and found on the left of the blade holder, to unlock the blade 
holder.   

2.  Adjust the blade holder that now moves to the left and right. 
3.  When the adjustment is complete, tilt away from you the blade 

holder left/right lever shown in yellow, to lock the blade holder.   

 
 
 

 

 

 

 

If the blade holder stops moving to the left and right, tilt all the 
way toward you the blade holder left/right lever with a yellow 
ring, to remove and clean the lever. 
Refer to p. 3-7 for the cleaning method. 

 

The black handle of each lever on the blade holder can be set at 
any angle. 
After the lever is set to the lock position, pull the handle 
outward [1] and turn it to a desired position [2]. Release the 
handle to lock it. 

 

 

 

 
 

Lever (yellow) (Blade holder left/right lever)

 

Left position

 

Normal position

 

Right position

 

Black lever handle 

Содержание Tissue-Tek Polar DM

Страница 1: ...Operating Manual Frozen Tissue Section Preparation Instrument Cryostat Microtome...

Страница 2: ...nt cryostat microtome Tissue Tek Polar herein after simply referred to as the Polar instrument is an instrument to section frozen tissues Carefully read this Operating Manual and use the Polar instrum...

Страница 3: ...e Right Panel DM only 1 11 How to Operate the Right Panel for Motorized Sectioning 1 11 Starting Motorized Sectioning 1 11 Pausing Motorized Sectioning 1 11 Stopping Motorized Sectioning 1 11 Adjustin...

Страница 4: ...Setting Screen 1 39 Setting the Cleaning Operation Defrost Cycle Ozone cycle Start Time 1 40 Explanation of Scheduled Operation Setting Screen 1 41 Setting the Operation Ready Shutdown Times 1 42 Set...

Страница 5: ...l Sectioning Final Shaving 2 11 Press the home position key to move the specimen head to the sectioning start position 2 11 Cutting the Specimen to Flat Surface Rough Shaving 2 11 Checking the Section...

Страница 6: ...r 2 36 Plastic Object Holder 2 37 Using the Plastic Object Holder 2 37 Adapter for Cryo3 Object Holder 2 38 Using Adapter for Cryo3 Object Holder 2 38 Freeze the Block Using the PINO Hyfluid Method 2...

Страница 7: ...Intake Grille 3 12 Replacement of the Brine Solution auxiliary cooling fluid 3 12 Chapter 4 Troubleshooting Troubleshooting 4 1 Before Suspecting a Trouble Instrument Conditions and Remedial Actions...

Страница 8: ...roperty or negatively affect the processing result Point to note or other useful information Specifications and other details of the Polar instrument are subject to change without notice for the reaso...

Страница 9: ...eavy objects on the power cord and store it in a narrow place Doing so may damage the power cord Do not place the instrument in a location exposed to direct sunlight snow or rain Ultraviolet rays and...

Страница 10: ...ly causing fire accident failure etc Do not directly touch with hands etc those areas whose temperature drops to low levels Never directly touch with hands etc the chamber and other locations subject...

Страница 11: ...Wear a mask gloves goggles and other protective gears while working or cleaning The Polar instrument has a blade and glass edges to demonstrate its characteristics Since these parts may cause injury...

Страница 12: ...of power outage so the tissue sample may melt Also the disposable blade may frost and rust Do not leave the tissue sample and disposable blade in the chamber Set the chamber temperature and specimen h...

Страница 13: ...ra distributor or representative If an error that cannot automatically be recovered occurs there might be ozone still inside the chamber Also even if an error can automatically be recovered to be extr...

Страница 14: ...while the instrument is not in use If the instrument will not be used for an extended period of time be sure to close the window Otherwise the cooling part in the chamber may frost and cause the opera...

Страница 15: ...Chapter 1 Basic Knowledge of Instrument...

Страница 16: ...home position key 1 trim key etc let you section tissues in fewer steps The wide chamber also allows for smooth collection of sections Interior walls of the chamber are flat for easy cleaning 1 The h...

Страница 17: ...ty The ambient operating temperature range of the Polar instrument is 10 to 35 C The ambient operating humidity range is 30 to 85 relative humidity non condensing Locate the instrument in a well venti...

Страница 18: ...h This switch is used to stop the microtome operation Refer to p 1 13 for details 5 Right panel DM only Motorized sectioning can be started or stopped and the sectioning speed adjusted on this panel a...

Страница 19: ...e levels The air volume can be checked with the center indicator lamp Refer to p 2 34 for details 16 Draft generator filter LEDs The two LEDs tell you when to replace the HEPA filter in the optional d...

Страница 20: ...and the specimen head continues to advance while the key is held 5 TRIM key Turn the handwheel while holding this key to section the tissue to flat surface at a desired trim thickness according to th...

Страница 21: ...is implement is used to prevent the section from curling Refer to p 2 13 for details 7 Anti Roll Plate This implement is used to prevent the section from curling Refer to p 2 14 for details 8 Blade ho...

Страница 22: ...chamber To be specific the two move as follows When the handwheel is turned by one revolution from the condition in 1 to the condition in 4 the specimen head comes down 2 and the tissue is sectioned...

Страница 23: ...er away from you The handwheel is locked when the specimen head is at the top position The handwheel is not locked if the specimen head is at any other position Note that turning the handwheel with th...

Страница 24: ...to the movement of the specimen head during motorized sectioning Use the pause key on the right panel to unlock the foot switch Refer to p 1 10 for details Connecting the Foot Switch Connect the foot...

Страница 25: ...or representative TYPE 1 Step on the foot switch once to start motorized sectioning Step on the foot switch again to start motorized sectioning TYPE 2 Hold the foot switch and the instrument continue...

Страница 26: ...set each mode Refer to p 1 35 for information on how to set the sectioning end position Press the pause key and stop key together to start sectioning Do not turn the handwheel while sectioning is pau...

Страница 27: ...he Cryomold The standard round type and square type have a knob for easy separation of the frozen tissue sample from the Cryomold Type Explanation Square type standard 26 x 31 mm Optional Used for sta...

Страница 28: ...s the emergency stop switch EMERGENCY STOP appears on the screen and the motor stops immediately 2 Lock the handwheel to protect the tissue samples and then turn off the power switch of the instrument...

Страница 29: ...toward you 2 Hold the knob on the blade guard and tilt it away from you to guard the tip of the disposable blade 3 To store the blade guard perform steps 1 and 2 in the reverse order The Anti Roll Pla...

Страница 30: ...rred or otherwise the power was cut off during processing and the processing stopped recovery processing is performed once the system recovers The following operations are performed Defrost Manual ozo...

Страница 31: ...e holder 20 mm from the specimen mounting base Sectioning auxiliaries Anti Roll Plate Anti Roll Rake Defrost cycle Hot gas heater Anti infection functions Ozone disinfection cycle Approx 75 minutes in...

Страница 32: ...1 17 Standard Accessories Options 4 5 15...

Страница 33: ...Anti Roll Rake F60 691 00 1 set 2 Anti Roll Plate F60 953 00 1 set 3 Blade holder D DM type F60 647 00 1 set 4 Heat extractor standard F60 622 00 1 unit 5 Parking plate standard O86 649 03 1 unit 6 Tr...

Страница 34: ...TION Tab Manual motorized sectioning information and operations are displayed set and performed Refer to p 1 21 for details MOTORIZED Tab DM only Motorized sectioning information and operations are di...

Страница 35: ...e cooling Once the cooling is started the snowflake icon changes as follows A short animation showing that chamber is being cooled is played switching from the standby mode and once the cooling temper...

Страница 36: ...e temperature in the chamber The new temperature will also apply during motorized sectioning Refer to p 1 32 for details Specimen head temperature setting button Press this button to change the temper...

Страница 37: ...and switch to the Temperature Setting 1 Temperature Setting 2 or FREE mode Section thickness setting button Press this button to change the section thickness to be used when sectioning Refer to p 1 30...

Страница 38: ...and Press the Motorized Sectioning Mode Selection button to change the mode TRIM Mode Screen In the TRIM mode rough shaving is performed to the preset feed amount TRIM thickness While in the TRIM mode...

Страница 39: ...rature Setting 1 2 mode Motorized sectioning mode selection button Press this button to select and switch to the TRIM FINE or PAUSE mode Section thickness setting button Press this button to change th...

Страница 40: ...Tab become effective To change these temperatures change them in the FREE mode or Temperature Setting 1 2 mode Motorized sectioning mode selection button Press this button to select and switch to the...

Страница 41: ...ng button You can set how much to offset the specimen head home position when moving to the home position following the pressing of the home position key Refer to p 1 34 for details Temperature settin...

Страница 42: ...t TRIM Thickness and Draft Generator Refer to p 1 49 for the details of permissions Defrost button Start the defrost cycle manually as desired Refer to p 2 18 for details Shutdown button Start shutdow...

Страница 43: ...GE button You can change the language Refer to p 1 46 for details Maintenance button Display maintenance information Refer to p 1 46 for details Cooling method button You can select Priority on Head T...

Страница 44: ...be pressed when the first or last page of the list is displayed Refer to p 1 51 for details Output button This button can be pressed when a USB memory device is connected so that the error log list a...

Страница 45: ...t saved and the screen closes automatically How to Change in the Motorized Sectioning Mode The section thickness can be changed only when the motorized sectioning mode is FINE or PAUSE If TRIM is sele...

Страница 46: ...ectioning 1 Press the TRIM Thickness Setting button on the UTILITIES Tab Screen 2 The TRIM thickness change screen appears 3 Press the buttons among the Value Change buttons to change the value of sec...

Страница 47: ...b Screen 2 The specimen head temperature setting screen appears The screen shows the measured value current temperature and setting value temperature to be set 3 Press the buttons among the Value Chan...

Страница 48: ...to change and the value will be selected and highlighted 4 To change the chamber temperature press the value in the chamber temperature icon Press the buttons among the Value Change buttons to change...

Страница 49: ...saves the setting after which the display returns to the TUNING Tab Screen If no operation has been performed for 5 minutes the setting is not saved and the screen closes automatically 5 Physically p...

Страница 50: ...d to the setting position where you want to stop the specimen head As the handwheel rotates the specimen head in the chamber moves according to the value As long as the value is in the settable range...

Страница 51: ...esired Suction Force 1 Press the Suction Force Setting button on the TUNING Tab Screen 2 The suction adjustment screen appears 3 Press the buttons among the Value Change buttons to change the value of...

Страница 52: ...in the date selection area and the set date time is updated Set button Press this button to change the time Pressing this button displays the time change screen Save button Save the setting After the...

Страница 53: ...Set button 4 Set the hours and minutes Press the value you want to change and the value will be selected and highlighted 5 To change the hours press the value of hours Press the buttons among the Valu...

Страница 54: ...cannot press these buttons Refer to p 1 45 for details Exit button Return to the UTILITIES tab screen Automatic ozone cycle check box button If the check box is selected the ozone cycle will be perfo...

Страница 55: ...r display hours is displayed in a range of 1 to 12 and minutes as a multiple of 15 minutes with am pm also shown 7 Pressing the Enter button saves the setting after which the display returns to the UT...

Страница 56: ...to input output schedule data Note that the Everyone user cannot press these buttons Refer to p 1 45 for details Exit button Return to the UTILITIES tab screen Automatic ozone cycle check box button...

Страница 57: ...ress the value of hours 6 Press the buttons among the Value Change buttons to change the value of hours In the case of 24 hour display Hours is displayed in a range of 0 to 23 and minutes as a multipl...

Страница 58: ...switch to the previous month or button to switch to the next month The settable range is January 2000 through December 3000 5 Pressing the Save button closes the setting screen Deleting Days Off Setti...

Страница 59: ...to switch to the next month The settable range is January 2000 through December 3000 5 Pressing the Save button closes the setting screen If a given day is set as both a day off and operating day it i...

Страница 60: ...screen schedule displays the file transfer confirmation screen 2 Press Yes and the schedule information backed up to the CF card in the instrument will be loaded The currently set information will be...

Страница 61: ...ance information is displayed Cumulative ozone lamp ON hours Cumulative compressor operating hours Cumulative Cryo operating hours 4 Press the close button to close the screen Setting the Cooling Meth...

Страница 62: ...sound by pressing the Test button 4 Pressing the Save button saves the setting and closes the setting screen Changing the Sound 1 Press the Miscellaneous button on the UTILITIES Tab Screen 2 Press the...

Страница 63: ...button displays the password setting screen 5 Use the numeric keypad to enter a password in the yellow password entry field Up to 6 digits can be entered The numbers from 1 to 999999 can be used but a...

Страница 64: ...ature setting 1 32 1 33 Temperature setting mode change 1 21 Section thickness setting 1 30 MOTORIZED Tab Temperature setting mode change 1 24 Section thickness setting 1 30 End of section position 1...

Страница 65: ...store button on the external input output screen password displays the file transfer confirmation screen 2 Press Yes and the password information backed up to the CF card in the instrument will be loa...

Страница 66: ...ntly displayed press the Log List Switching button to switch to the event log 3 The event log list display area shows each event number and relevant information such as the Date Time on which the even...

Страница 67: ...he software To update the software contact the nearest authorized Sakura distributor or representative Checking the Version 1 Press the Software button on the UTILITIES Tab Screen 2 Press the Exit but...

Страница 68: ...1 53...

Страница 69: ...Chapter 2 Operating Method...

Страница 70: ...specimen head Adjust the specimen head Change the disposable blade Preparing for Sectioning Details p 2 4 Move the specimen head to the sectioning start position Cut the specimen to flat surface rough...

Страница 71: ...r switch is found on the right side face of the Polar instrument Tilt the switch upward to turn on the power When the power is turned on the startup screen appears and shows the progress of startup pr...

Страница 72: ...m the Sleep Mode If the screen is in the sleep mode press the Wake up button on the screen to switch to the password entry screen If the screen remains dark and nothing is displayed touch the screen o...

Страница 73: ...ng the Specimen Head Temperature 1 Press the Specimen Head Temperature Setting button on the SECTION Tab Screen 2 The specimen head temperature setting screen appears 3 Press the buttons among the Val...

Страница 74: ...een to start rapid cooling Continue cooling until the Cryo temperature drops to 50 C or below Cooling will continue for 15 minutes after the button is pressed To stop cooling press the Cryo button aga...

Страница 75: ...en head 4 Refer to p 2 7 if the specimen head angle must be adjusted If compound etc is deposited on the installation surface or tapered part of the Object Holder or to the specimen head the Object Ho...

Страница 76: ...ead up Angle adjustment locking lever This lever locks the vertical angle adjustment screw and lateral angle adjustment screw Raise the lever toward you to unlock the screws so that the angles can be...

Страница 77: ...ently installed Refer to p 2 10 for details 4 Insert the new disposable blade between the blade holder and stage 2 5 Push out the old disposable blade if it still remains 3 6 Use the handle of a brush...

Страница 78: ...te tilt away from you the blade holder left right lever shown in yellow to lock the blade holder If the blade holder stops moving to the left and right tilt all the way toward you the blade holder lef...

Страница 79: ...3 7 Tilt away from you the disposable blade mounting lever indicated in red to lock the disposable blade 4 The Low Profile type refers to A35 S35 and C35 disposable blades among others How to Set the...

Страница 80: ...imen head moves further The home position may not be appropriate depending on the height or orientation angle of the frozen block Turn the handwheel slowly to check the home position Press the home po...

Страница 81: ...specimen by flattening the section using a brush etc It is recommended that you collect the fifth and subsequent sections because the first three or four sections tend to have unstable thickness due...

Страница 82: ...ctioning and the tip of the Anti Roll Rake will be coupled and move forward on the stage by pulling the section 2 Remove the Anti Roll Rake by holding the section with a brush etc 3 Once a section is...

Страница 83: ...the Anti Roll Plate and the stage return the Anti Roll Plate to its original position 4 Slowly move the glass slide closer to the section from above and the section will be attached to the glass slid...

Страница 84: ...and pressing it again switches the vacuuming to the desired suction force Press the key one more time and suctioning will end If the suction force is currently set to 100 on the TUNING Tab Screen pres...

Страница 85: ...cancelled Cleaning operation will be performed so long as all conditions are satisfied such as no operation for 30 minutes during the day 3 Cleaning operation starts and the cleaning operation progre...

Страница 86: ...ion ends while this screen is displayed the screen closes automatically This password entry screen is for aborting the cleaning operation not for logging on the system 3 Select Abort and defrost cycle...

Страница 87: ...n and the defrost operation cancellation confirmation screen will appear For details refer to the next item Cancelling Manual Defrost Cycle Do not leave the chamber with frozen blocks placed inside Th...

Страница 88: ...peration cancellation confirmation screen will appear For details refer to the next item Cancelling Manual Ozone Cycle If alcohol was used in the chamber or waste tank dry the chamber tank completely...

Страница 89: ...up time setting screen to stop cooling operation make the instrument sleep until the start date time after cleaning operation If Always on operation is selected for the schedule cleaning operation is...

Страница 90: ...ar and remaining time If the check box for automatic ozone cycle is selected in the schedule the ozone cycle will be performed after defrost cycle Once the processing is complete the display changes t...

Страница 91: ...ly after which the screen will close If ozone cycle is also set as part of the cleaning operation the shutdown cancellation screen will appear and ozone cycle will be cancelled However the ozone purge...

Страница 92: ...t the automatic shutdown time 2 If Yes is selected on the automatic shutdown confirmation screen or the displayed wait time 5 minutes becomes 0 the instrument is automatically logged off and automatic...

Страница 93: ...le this screen is displayed the screen closes automatically This password entry screen is for entering the password to cancel the processing so the instrument need not be logged on 3 Select Abort and...

Страница 94: ...n the left shows an example of an error occurring during manual ozone disinfection A screen is displayed to inform the operator that ozone disinfection might have been insufficiently performed as a re...

Страница 95: ...otorized sectioning Press the Motorized Sectioning Mode Change button on the MOTORIZED Tab Screen to change the mode TRIM mode In the TRIM mode you can automatically cut the specimen to flat surface r...

Страница 96: ...every time each key is pressed Press and hold the key and the specimen head moves further Cutting the Specimen to Flat Surface Rough Shaving in the TRIM Mode Press the Motorized Sectioning Mode Chang...

Страница 97: ...the MOTORIZED Tab Screen is currently not displayed or the handwheel is not yet unlocked an error sound is output and motorized sectioning cannot be started Besides manual sectioning you can use the...

Страница 98: ...oll Plate falls sharply its front end may get contact with the disposable blade and broken This may lead to scatter of glass fragment and cause injury The broken Anti Roll Plate may also interfere wit...

Страница 99: ...s Lever yellow Blade holder left right lever Unlock this lever to slide the blade holder to adjust its lateral position Refer to p 2 9 for details Lever blue Blade holder mounting lever Unlock this le...

Страница 100: ...n wrench away from you the blade angle adjustment bolt found on the left side face of the blade holder The recommended angle relief angle stamped on the blade holder is 8 to 10 Once the position is fi...

Страница 101: ...pter is used Do not put the blade holder mounting lever indicated in blue in the wrong hole 1 Set the blade holder over the blade holder locking projections in the chamber Make sure the top face of th...

Страница 102: ...sert the metal plug into the normally used locking hole 3 put the screw into the threaded hole into which the metal plug was inserted and tighten the screw 4 3 Set the blade holder over the blade hold...

Страница 103: ...erate the vacuum press the vacuum key on the left panel When a given suction force is currently set pressing this button once starts suctioning at 100 and pressing it again switches the vacuuming to t...

Страница 104: ...the setting is not saved and the screen closes automatically Stopping the Draft Generator 1 Press the Draft Generator button on the UTILITIES tab screen 2 The draft generator setting screen appears 3...

Страница 105: ...heat extractor at the tip of the arm installed on the Cryobar rail in the chamber 1 2 Put the heat extractor on the parking plate 2 3 Press the Cryo button among the buttons on the right side of the...

Страница 106: ...ic Object Holder that does not conduct heat effectively Put tissue specimens in the Cryomold Put OCT compound by making sure it does not overflow Put the plastic Object Holder on top Make sure the sid...

Страница 107: ...bject Holder is used Freeze the Block Using the PINO Hyfluid Method Frozen blocks can be prepared in approx 45 seconds using the PINO Hyfluid method Incompletely frozen blocks can also be cooled again...

Страница 108: ...2 39...

Страница 109: ...Chapter 3 Maintenance Inspection...

Страница 110: ...e chamber to room temperature Wipe out disinfectant residue using a cloth damp with water and dry the chamber interior thoroughly If a disinfectant such as glutaraldehyde remains it may cause health h...

Страница 111: ...ast 1 hour in 2 glutaraldehyde After the soak thoroughly rinse the tank under running water and dry it before setting in the instrument Wear a mask gloves goggles and other protective gears when handl...

Страница 112: ...ct the connection port on the vacuum unit to the instrument side connection port in the chamber 1 Using the connected part 1 as the axis put the vacuum filter set on the vacuum filter setting base 2 2...

Страница 113: ...instrument Refer to p 3 3 for the installation method Cleaning the Vacuum Hose If debris attach inside the vacuum hose its suction capacity will drop Clean the drain periodically Besides disinfectant...

Страница 114: ...mality the LED lamp and suction level lamp start blinking turn to steady light to notify the situation The lamp modes and meanings are listed below If the particular situation you encountered is not l...

Страница 115: ...bring it to a safety cabinet etc 4 Spray disinfectant alcohol on the draft generator placement area on the back of the instrument 5 Take out the draft generator from the bio hazard bag inside the safe...

Страница 116: ...ethod from the chamber 2 Disassemble the moving part that slides to the left and right carrying the stage Tilt toward you the blade holder left right lever indicated in yellow to unlock the blade hold...

Страница 117: ...ve washer spring and lever locking bracket for securing the stage in the chamber are set If the wave washer is not inserted the blade holder cannot be locked and sectioning won t be performed Be caref...

Страница 118: ...t smoothly If not the right and left mounting plates are out of line Set the mounting plate so that it is parallel with the blade holder to avoid hitting it How to Install the Anti Roll Plate 1 If the...

Страница 119: ...hten the glass locking screws Both top and bottom edges of the glass plate of the Anti Roll Plate can be used It is recommended to turn the glass plate upside down after one edge has been used for abo...

Страница 120: ...tective gears when handling a disinfectant such as glutaraldehyde Thoroughly rinse the object holder with water after disinfected and dry it If a disinfectant such as glutaraldehyde remains it may cau...

Страница 121: ...r or something Replacement of the Brine Solution auxiliary cooling fluid The brine solution must be replaced on a regular basis If the brine solution continues being used even after its replacement ti...

Страница 122: ...3 13...

Страница 123: ...Chapter 4 Troubleshooting...

Страница 124: ...y 3 3 Isn t the desired suction force set low Raise the set value of desired suction force 1 36 The chamber cannot be cooled Isn t the setpoint temperature high Readjust the setpoint temperature 1 21...

Страница 125: ...n of the handwheel Follow the instructions below to lessen the noise although it does not interfere with instrument operations 1 Loosen the vent cap to remove 2 Perform the automatic cleaning operatio...

Страница 126: ...solve the problem 1 Remove the blade holder temporarily 2 Confirm the handwheel is locked 3 Raise the specimen head locking lever to place and lock the object holder on the specimen holder 4 Using th...

Страница 127: ...This information is displayed immediately after the instrument is started and also after returning from the sleep mode If an unexpected problem occurred and the condition cannot be rectified easily tu...

Страница 128: ...ile error occurred while the software was being updated Restart the instrument If the problem persists contact the Sakura authorized distributor 200 ABNORMAL CHAMBER SENSOR READING LOW TEMP Disconnect...

Страница 129: ...horized distributor 360 WINDOW LOCK ERROR Unable to Lock the Window Open the window fully and then close the window If the problem persists contact the Sakura authorized distributor 361 WINDOW UNLOCK...

Страница 130: ...e moved to the preset sectioning start position Log on log off Limit the instrument user by password entry The instrument is logged on when the password has been entered and logged off when the passwo...

Страница 131: ...the instrument been used in a room where is in charge of pathology of infectious diseases Yes No 4 Has the instrument been used in a pathology laboratory for infectious diseases Yes No 5 Has the instr...

Страница 132: ...ven during the warranty period 1 Malfunction and damage caused by a wrong handling 2 Malfunction and damage caused by a repair disassembly or modification performed by a party other than Sakura or Sak...

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